詳細(xì)說明
概述
產(chǎn)品名稱Anti-NR0B1 / Dax1抗體[EP13786] - N-terminal
描述
兔單克隆抗體[EP13786] to NR0B1 / Dax1 - N-terminal
經(jīng)測試應(yīng)用ICC/IF,IHC-P,WB
種屬反應(yīng)性
與反應(yīng): Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NR0B1/ Dax1 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P51843陽性對照
A431 and Human testis lysates; Human testis tissue; SH-SY5Y cells.
常規(guī)說明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb? technology. RabMAb? technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放說明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存儲溶液Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA純度Protein A purified
克隆單克隆
克隆編號EP13786
同種型IgG
研究領(lǐng)域
Developmental Biology
Reproduction
Placental development
Developmental Biology
Reproduction
Sex determination
Epigenetics and Nuclear Signaling
Nuclear Signaling Pathways
Nuclear Receptors
Orphan Nuclear Receptors
Epigenetics and Nuclear Signaling
Nuclear Signaling Pathways
Nuclear Receptors
Retinoic & Retinoid
Neuroscience
Endocrine system
Hypothalamic pituitary adrenal axis
Neuroscience
Endocrine system
Gonadotrophic axis
Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal 圖像
Immunocytochemistry/ Immunofluorescence - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)
Immunofluorescent analysis of SH-SY5Y cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling NR0B1 / Dax1 with ab196649 at 1/300 dilution followed by Goat anti rabbit IgG (AlexaFluor? 488) (ab150077) secondary at 1/400 dilution and counter-stained with DAPI (blue).
Note: Nuclear and cytoplasm staining on SH-SY5Y cell line was observed.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling NR0B1 / Dax1 with ab196649 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).
Note: Nuclear staining on human testis tissue was observed.
Western blot - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)
Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649) at 1/1000 dilution + Human testis lysate at 10 μg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 52 kDa
Western blot - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)
Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649) at 1/1000 dilution + A431 cell lysate at 20 μg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size : 52 kDa