詳細(xì)說(shuō)明
概述
產(chǎn)品名稱(chēng)Anti-Nuf2抗體[EPR13019]
描述
兔單克隆抗體[EPR13019] to Nuf2
經(jīng)測(cè)試應(yīng)用WB,IP
種屬反應(yīng)性
與反應(yīng): Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Nuf2 aa 450 to the C-terminus. The exact sequence is proprietary.
Database link: Q9BZD4陽(yáng)性對(duì)照
HeLa, A431 and 293 cell lysate.
常規(guī)說(shuō)明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb? technology. RabMAb? technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放說(shuō)明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存儲(chǔ)溶液Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA純度Protein A purified
克隆單克隆
克隆編號(hào)EPR13019
同種型IgG
研究領(lǐng)域
Cell Biology
Cell Cycle
Cell Division
Centromere
Anti-Nuf2 antibody [EPR13019] 圖像
![Western blot - Anti-Nuf2 [EPR13019] antibody (ab180945)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab180945451.jpg)
Western blot - Anti-Nuf2 [EPR13019] antibody (ab180945)
All lanes : Anti-Nuf2 antibody [EPR13019] (ab180945) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : 293 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 54 kDa
Blocking/ Dilution buffer: 5% NFDM /TBST.
![Immunoprecipitation - Anti-Nuf2 [EPR13019] antibody (ab180945)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab180945452.jpg)
Immunoprecipitation - Anti-Nuf2 [EPR13019] antibody (ab180945)
Lysate from 293 cells was immunoprecipitated with ab180945 at a 1/50 dilution. For the subsequent blot, ab180945 used at a 1/1000 dilution with Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST. Second lane is the negative control.
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