• Species Reactivity

  Human

• Specificity

  Detects CL?P1/COLEC12 in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross?reactivity with recombinant mouse CL-P1 is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human CL?P1/COLEC12 isoform 1    
  Leu57-Leu742    
  Accession # Q5KU26

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 μg/mL

  Recombinant Human CL-P1/COLEC12 (Catalog # )

• 
  

• Flow Cytometry

  2.5 μg/10    ⁶ cells

  See below

• 
  

• Blockade of Receptor-ligand Interaction

  In a functional ELISA, 2-8 μg/mL of this antibody will block 50% of the binding of 250 ng/mL of biotinylated AGE?BSA to immobilized Recombinant Human CL-P1/COLEC12 (Catalog # ) coated at 5 μg/mL (100 μL/well). At 100 μg/mL, this antibody will block >90% of the binding.

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

• Immunocytochemistry

  5-15 μg/mL

  Immersion fixed HUVEC human umbilical vein endothelial cells

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CL-P1/COLEC12

Collectins are a family of Ca⁺⁺-dependent, C-type lectins that contain a collagenous domain and function as recognition molecules for molecular patterns found on pathogens (1?4). Human collectin placenta 1 (CL-P1; also known as collectin sub-family member 12 and SRCL type I [scavenger receptor with C-type lectin type I]) is a 110 kDa member of the collectin family of glycoproteins (5, 6). CL-P1 is the only collectin known to be membrane bound, while CL-L1 (collectin liver-1) is the only known cytoplasmic collectin (1). Human CL-P1 is synthesized as a 742 amino acid (aa) type II transmembrane glycoprotein that contains an N-terminal 39 aa cytoplasmic domain, a 17 aa transmembrane segment, and a 686 aa C-terminal extracellular region (6). The short cytoplasmic domain contains an internalization motif (Y-K-R-F) while the extracellular region is complex, demonstrating a coiled-coil segment, a Ser-Thr rich region, a collagen-like structure and a C-type lectin/ carbohydrate recognition domain (CRD). Notably, this CRD recognizes galactose (and fucose) within the context of asialo?orosomucoids associated with the Lewis^x epitope (7, 8). CL-P1 has a 300 kDa trimeric form due to its collagen-like and coiled-coil helical domains (1, 5). There is a 97 kDa, alternate splice form of CL-P1 (SRCL type II) that shows a 120 aa truncation at the C-terminus. This effectively removes the entire CRD found on full?length CL-P1 (6). Human CL-P1 is 93% aa identical to mouse CL-P1 over the entire extracellular region, and 87% aa identical over the CRD region (5, 9). Human CL-P1 is known to be expressed in vascular endothelial cells (5).

• References:

1. van de Wetering, JK. et al. (2004) Eur. J. Biochem. 271:1229.

2. Holmskov, U. et al. (2003) Annu. Rev. Immunol. 21:547.

3. Hoppe, H-J. and K. Reid (1994) Protein Sci. 3:1143.

4. Hickling, T.P. et al. (2004) J. Leukoc. Biol. 75:27.

5. Ohtani, K. et al. (2001) J. Biol. Chem. 276:44222.

6. Nakamura,K. et al. (2001) Biochem. Biophys. Res. Commun. 280:1028.

7. Coombs, P.J. et al. (2005) J. Biol. Chem. 280:22993.

8. Yoshida, T. et al. (2003) J. Biochem. 133:271.

9. Nakamura, K. et al. (2001) Biochim. Biophys. Acta 1522:53.

• Long Name:

  Collectin Placenta 1

• Entrez Gene IDs:

  81035 (Human); 140792 (Mouse); 361289 (Rat)

• Alternate Names:

  CL-P1; CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae); COLEC12; EC 2.7.1.78; hClp1; HEABATP/GTP-binding protein; homolog of yeast CFIA subunit Clp1p; NSR2; Polynucleotide kinase Clp1; polyribonucleotide 5'-hydroxyl-kinase Clp1; Pre-mRNA cleavage complex II protein Clp1; SCARA4; SRCL Type I