• Species Reactivity

  Human

• Specificity

  Detects human GASP-2/WFIKKN in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human GASP-1 is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 289827

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human GASP-2/WFIKKN    
  Ala20-Asp548    
  Accession # Q96NZ8

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Human GASP-2/WFIKKN Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  2-8 μg/mL 

  Human GASP?2/WFIKKN Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.5-2.0 μg/mL 

  Human GASP?2/WFIKKN Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Human GASP-2/WFIKKN Protein (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GASP-2/WFIKKN

Growth and differentiation factor-associated serum protein-2 (GASP-2) cDNA encodes a 548 amino acid protein that contains a 19 amino acid signal sequence and is comprised of many conserved domains: WAP, follistatin/Kazal, immunoglobulin, two tandem Kunitz, and netrin (1). Based on the order of these conserved domains, GASP-2 is also known as WFIKKN (1). Another related protein which contains the same domain structure is called WFIKKNRP (WFIKKN-related protein), or GASP-1 (2, 3). WAP, follistatin, Kunitz and netrin domains are all implicated in protease inhibition, and the GASP proteins may be multivalent protease inhibitors (1, 4). Tests at R&D Systems have measured the ability of GASP-2 to inhibit trypsin cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH₂ (R&D Systems Catalog # ES002). The IC₅₀ value was approximately 10 nM, as measured in a reaction mixture containing 1.0 nM trypsin, 10 μM ES002, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, pH 7.5.

GASP-1 and -2 show distinct expression patterns both in the developing fetus and the adult. In the developing fetus, GASP-2 is abundant in the lung, skeletal muscle and liver while GASP-1 expression is highest in the brain, skeletal muscle, thymus and kidney (3). In the adult, GASP-2 is expressed primarily in the pancreas, liver, and thymus while GASP-1 is in the ovary, testis, and brain (3). Further characterization shows that GASP-1 inhibits myostatin (GDF-8) and the highly related protein, GDF-11, but not Activin or TGF-beta in vitro (2). Although, this kind of activity has not been reported for GASP-2, tests at R&D Systems have determined that GASP-2 shows similar inhibitory activity towards myostatin as GASP-1. By amino acid sequence, human GASP-2 is 55% identical to human GASP-1.

• References:

1. Trexler, M. et al. (2001) Proc. Natl. Acad. Sci. USA 98:3705.

2. Hill, J.J. et al. (2003) Mol. Endo. 17:1144.

3. Trexler, M. et al. (2002) Biol. Chem. 383:223.

4. Nagy, A. et al. (2003) Eur. Jour. Biochem. 270:2101.

• Long Name:

  Growth and Differentiation Factor-associated Serum Protein-2

• Entrez Gene IDs:

  117166 (Human)

• Alternate Names:

  C16orf12; Chromosome 16 Open Reading Frame 12; GASP2; GASP-2; Growth And Differentiation Factor-Associated Serum Protein 2; hGASP-2; RJD2; WAP Four-Disulfide Core Domain 20A; WAP, Follistatin/Kazal, Immunoglobulin, Kunitz And Netrin Domain Containing 1; WAP, FS, Ig, KU, And NTR-Containing Protein; WFDC20A; WFIKKN; WFIKKN1