• Species Reactivity

  Human

• Specificity

  Detects recombinant human VIP in direct ELISAs. It does not detect the C-terminal region (aa 145-152).

• Source

  Monoclonal Mouse IgG    ₁ Clone # 576721

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  KLH-coupled human VIP synthetic peptides HSDAVFTDNYTR and KYLNSILN

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Immunohistochemistry

  8-25 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VIP

Vasoactive intestinal peptide (VIP) is a 28 amino acid (aa) peptide that exerts wide-ranging effects in embryonic and adult tissues through interactions with the 7?transmembrane spanning receptors VIP R1 and VIP R2. The VIP proprotein is proteolytically processed to release bioactive VIP as well as the bioactive peptides PHV and PHM. VIP regulates appetite and feeding behavior and stimulates insulin and glucagon secretion in pancreatic islet cells. It also regulates astrocyte function, multiple aspects of cardiac function, and TLR function in innate immune responses. Mature VIP shares 100% aa sequence identity with mouse and rat VIP.

• Long Name:

  Vasoactive Intestinal Peptide

• Entrez Gene IDs:

  7432 (Human)

• Alternate Names:

  MGC13587; PHM27; vasoactive intestinal peptide; VIP peptides; VIP