• Species Reactivity

  Human

• Specificity

  Detects human Progesterone R B in Western blots.

• Source

  Monoclonal Mouse IgG    _2B Clone # 569012

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Progesterone R B    
  Met1-Leu189    
  Accession # P06401

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  See below

• 
  

• Immunohistochemistry

  5-25 μg/mL

  See below

• 
  

• Immunocytochemistry

  3-25 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Progesterone R B/NR3C3

Progesterone Receptor B (PR-B) is a 118 kDa member of the NR3 subfamily within the nuclear hormone receptor family of proteins. It is expressed in several tissues, including female reproductive organs as well as neurons throughout the CNS. PR-B is particularly important in the mammary gland where it mediates proliferative responses to progesterone. Human PR-B contains an N-terminal regulatory region (aa1?566), a DNA binding domain (aa 567?639), and a steroid-binding region (aa 681?933). Ligand binding induces a key phosphorylation event at Ser294 by ERK1/2.

• Long Name:

  Progesterone Receptor B

• Entrez Gene IDs:

  5241 (Human)

• Alternate Names:

  PGRB; Progesterone R B; Progesterone RB