• Species Reactivity

  Human

• Specificity

  Detects human Collagen IV alpha 1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Collagen I alpha 1, III alpha 1, XIII alpha 1v4, XXIII alpha 1, or recombinant mouse Collagen XXV alpha 1 is observed.    
   

• Source

  Monoclonal Mouse IgG    ₁ Clone # 577238

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Collagen IV alpha 1    
  Ser1441-Thr1669    
  Accession # P02462

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Immunocytochemistry

  8-25 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Collagen IV alpha 1

COL4A1 (collagen IV alpha 1) is a 185 kDa member of the type IV collagen family.  It is a secreted glycoprotein that is expressed by multiple cell types, including fibroblasts, keratinocytes and endothelial cells.  Two COL4A1 molecules interact with a 170 kDa alpha 2 chain to form a collagen IV triple helix.  This helix further interacts with other helices to generate covalent oligomers that form a scaffold in the basement membrane.  Mature human COL4A1 is 1642 amino acids (aa) in length.  It has an N-terminal “7S” proregion (aa 28-172), a central collagenous domain that contains multiple Gly-based repeats (aa 173-1440), and a C-terminal domain that is proteolytically cleaved to generate a 25-28 kDa NC1 globular segment that has potent antiangiogenic activity (aa 1441-1669).  Multiple splice forms exist.  One shows a deletion of aa 499-849, a second shows a seven aa substitution for aa 513-1669, and a third shows a seven aa substitution for aa 958-1669.  Over aa 1441-1669, human COL4A1 is 97% aa identical to mouse COL4A1.

• Entrez Gene IDs:

  1282 (Human); 12826 (Mouse); 290905 (Rat)

• Alternate Names:

  alpha-1 chain; arresten; COL4A1 NC1 domain; COL4A1; collagen alpha-1(IV) chain; Collagen IV alpha 1; collagen IV, alpha-1 polypeptide; collagen, type IV, alpha 1; Collagen-4; EC 3.4.23; EC 6.3.1.2