• Species Reactivity

  Human

• Specificity

  Designed to visualize the expression of LAMP1 by fluorescence microscopy for staining cells and tissues. Conjugated LAMP1 antibodies are ideal for immunocytochemistry colocalization studies in lysosomes. The unconjugated antibody detects human LAMP1/CD107a Lumenal Domain in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse LAMP1 is observed.

• Source

  Polyclonal Sheep IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human LAMP1/CD107a    
  Ala28-Asn380    
  Accession # P11279

• Formulation

  Supplied in a 10X saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 488

Applications

• Recommended    
  Concentration

  Sample

• Immunocytochemistry

  1:10 dilution

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: LAMP-1/CD107a

LAMP1 (Lysosome-associated membrane protein-1; also CD107a) is a 100?130 kDa member of the LAMP family of glycoproteins. It is expressed in lysosomal and plasma membranes of macrophages, NK and T-cells, and with LAMP2, is essential for the formation of phagolysosomes. On the cell surface, it also presents carbohydrates to selectins. Mature human LAMP1 is a 389 amino acid (aa) type I transmembrane glycoprotein. It contains a 354 aa luminal/extracellular domain (ECD) (aa 28?381) and a 12 aa cytoplasmic tail (aa 405?416). The ECD has two large looping regions (aa 28?193 and 227?381) plus multiple N- and O-linked glycosylation sites. There is one potential splice variant that shows a 26 aa substitution in the signal sequence. Over aa 28?380, human LAMP1 shares 64% aa identity with mouse LAMP1.

• Long Name:

  Lysosome-associated Membrane Glycoprotein 1

• Entrez Gene IDs:

  3916 (Human); 16783 (Mouse)

• Alternate Names:

  CD107 antigen-like family member A; CD107a antigen; CD107a; LAMP1; LAMP-1; LAMPA; LGP120; lysosomal-associated membrane protein 1; lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane protein 1