• Species Reactivity

  Human

• Specificity

  Detects human DEP-1/CD148 in Western blots.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 143-41

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Phytohemagglutinin-stimulated human peripheral blood mononuclear cells

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  See below

• 
  

• Flow Cytometry

  2.5 μg/10    ⁶ cells

  Human peripheral blood lymphocytes    
   

• 
  

• Immunoprecipitation

  2.5 μg/500 μg cell lysate

  HeLa human cervical epithelial carcinoma cell line,

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DEP-1/CD148

Density Enhanced Protein Tyrosine Phosphatase (DEP-1), also known as CD148, HPTP-eta, and PTP receptor type J (PTPRJ), is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. A large (220 kDa) glycoprotein found at the cell surface, DEP-1 levels are increased with high cell density (1). DEP-1 phosphatase activity is enhanced by basement membrane proteins (2), suggesting it is involved in regulating cell adhesion and contact interactions. High levels of expression dampen PDGF (3), VEGF (4), and T?cell receptor (5) responses. DEP-1 is widely expressed in tissues, particularly ones forming epithelioid monolayers (6). In the immune system, DEP-1 is found on all cell lineages and is highest on granulocytes (7). Dep-1 is the mutated gene in the Susceptibility to Colon Cancer locus Scc1, which is altered in many human colorectal adenomas (8). Gene knockout mice lacking DEP-1 die at midgestation due to failures in cardiovascular development (9). DEP-1 dephosphorylates a variety of proteins, including the HGF (10), PDGF (11), and VEGF (4) receptors, and beta-catenin (12). The recombinant protein is the intracellular region of DEP-1 containing the catalytic domain.

• References:

1. Ostman, A. et al. (1994) Proc. Natl. Acad. Sci. USA 91:9680.

2. Sorby, M. et al. (2001) Oncogene 20:5219.

3. Jandt, E. et al. (2003) Oncogene 22:4175.

4. Lampugnani, M.G. et al. (2003) J. Cell Biol. 161:793.

5. Baker, J.E. et al. (2001) Mol. Cell. Biol. 21:2393.

6. Borges, L.G. et al. (1996) Circ. Res. 79:570.

7. de la Fuente-Garcia, M.A. et al. (1998) Blood 91:2800.

8. Ruivenkamp, C.A. et al. (2002) Nat. Genet. 31:295.

9. Takahashi, T. et al. (2003) Mol. Cell. Biol. 23:1817.

10. Palka, H.L. et al. (2003) J. Biol. Chem. 278:5728.

11. Kovalenko, M. et al. (2000) J. Biol. Chem. 275:16219.

12. Holsinger, L.J. et al. (2002) Oncogene 21:7067.

• Long Name:

  Density Enhanced Receptor Protein Tyrosine Phosphatase

• Entrez Gene IDs:

  5795 (Human); 19271 (Mouse); 29645 (Rat)

• Alternate Names:

  CD148 antigen; CD148; Density-enhanced phosphatase 1; DEP1; DEP-1; DEP1EC 3.1.3.48; HPTP eta; HPTPeta; HPTP-eta; human density enhanced phosphatase-1; protein tyrosine phosphatase, receptor type, J polypeptide; protein tyrosine phosphatase, receptor type, J; Protein-tyrosine phosphatase eta; Protein-tyrosine phosphatase receptor type J; PTPRJ; receptor-type tyrosine-protein phosphatase eta; R-PTP-ETA; R-PTP-J; SCC1; susceptibility to colon cancer 1, mouse, homolog of