• Species Reactivity

  Human, Mouse

• Specificity

  Detects human and mouse Frizzled-7. In direct ELISAs, no cross-reactivity with recombinant mouse Frizzled-1, -3, -4 or -8 is observed.

• Source

  Monoclonal Rat IgG    _2A Clone # 151143

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-7    
  Gln33-Leu185    
  Accession # Q61090

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  2.5 μg/10    ⁶ cells

  Retinoic acid?treated D3 mouse embryonic stem cell line

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

• Immunocytochemistry

  8-25 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-7

Frizzled-7 is a member of the Frizzled family of unconventional G-protein-coupled glycoprotein receptors for the Wnt signaling pathway (1?3). The Wnt genes encode a large family of glycoproteins that are essential in development and tissue maintenance (1, 2). Like other Frizzled family members, Frizzled-7 contains a divergent N?terminal signal peptide (amino acid (aa) 1?32), a highly conserved extracellular cysteine-rich domain (CRD, aa 44?169), a variable-length linker region (aa 170?256), a seven-pass transmembrane region (aa 257?549), and a variable-length C-terminal cytoplasmic domain (aa 550?574) (1?3). The CRD, which comprises the binding site for Wnts and other ligands such as Syndecan 4 and fibronectin, spans about 130 amino acid residues and contains ten invariant cysteine residues (2, 3). Expressed alone, it can compete with native Frizzled to inhibit Wnt canonical signaling (4). Within aa 33?185, human Frizzled-7 shares ~99% aa identity with human, rat, canine and bovine Frizzled-7. Mature Frizzled-7 also shares 80% aa identity with Frizzled-1 and Frizzled-2. Roles for Frizzled-7 have been determined in both canonical Wnt/ beta ?Catenin-mediated signaling and non?canonical planar cell polarity and calcium pathways (1, 2, 4). During development, Frizzled-7 is expressed during gastrulation and in the fetal gut, kidney and lung where it is thought to influence tissue morphogenesis via non-canonical signaling pathways (3?5). In the adult, Frizzled-7 is expressed in skeletal muscle, especially in satellite cells that mediate muscle regeneration in response to Wnt-7a (3, 6). It is expressed in embryonic stem cells (ES), contributing to self-renewal signaling (7). It has been implicated in mesenchymal-to-epithelial transition in colorectal cancer (2, 8). Frizzled-7 mRNA has also been detected in adult heart and placenta (3).

• References:

1. Schulte, G. and V. Bryja (2007) Trends Pharmacol. Sci. 28:518.

2. Vincan, E. et al. (2007) Front. Biosci. 12:4558.

3. Sagara, N. et al. (1998) Biochem. Biophys. Res. Commun. 252:117.

4. Medina, A. et al. (2000) Mech. Dev. 92:227.

5. Kemp, C.R. et al. (2007) Dev. Dyn. 236:2011.

6. Le Grand, F. et al. (2009) Cell Stem Cell 4:535.

7. Melchior, K. et al. (2008) Biol. Chem. 389:897.

8. Vincan, E. et al. (2007) Oncogene 26:2340.

• Entrez Gene IDs:

  8324 (Human); 14369 (Mouse)

• Alternate Names:

  frizzled (Drosophila) homolog 7; frizzled homolog 7 (Drosophila); Frizzled, drosophila, homolog of, 7; Frizzled7; Frizzled-7; fz-7; FZD7; FzE3frizzled-7; hFz7