• Species Reactivity

  Human

• Specificity

  Detects human Laminin alpha 3/Laminin?5 in Western blots.

• Source

  Monoclonal Mouse IgG    ₁ Clone # P3H9-2

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Human keratinocytes

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  Wayner, E.A. (1993) J. Cell Biol.     121(5):1141; Gallatin, W.M.     et al. (1989) Proc. Natl. Acad. Sci. USA     86(12):4654. This application was not tested by R&D Systems.

• 
  

• Immunohistochemistry

  8-25 μg/mL

  See below

• 
  

• Immunoprecipitation

  Gallatin, W.M.     et al. (1989) Proc. Natl. Acad. Sci.     86:4654.

• 
  

• Adhesion Blockade

  Gonzales, M.     et al. (1999) Mol. Biol. Cell     10:259.

• 
  

• Immunocytochemistry

  8-25 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Laminin alpha 3/Laminin-5

Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. Through interactions with Integrins, Dystroglycan and other receptors, Laminins contribute to cell differentiation, cell shape and migration, and maintenance of tissue phenotypes and survival (1). Laminin-5, also known as Epiligrin, consists of an alpha 3, beta 3, and gamma 2 chain. It is abundant in transitional epithelium, stratified squamous epithelia, lung mucosa and other epithelial glands and plays an important role in the initiation and maintenance of epithelial cell anchorage to the underlying connective tissue (2, 3).

• References:

1. Colognato, H. et al. (2000) Dev. Dyn. 218:213.

2. Stahl, S. et al. (1997) J. Cell Sci. 110:55.

3. Gonzales, M. et al. (1999) Mol. Biol. Cell 10:259.

• Entrez Gene IDs:

  3909 (Human)

• Alternate Names:

  Epiligrin; LAMA3; Laminin alpha 3; Laminin5; Laminin-5