• Species Reactivity

  Human

• Specificity

  Detects human BTK when phosphorylated at Y551.

• Source

  Monoclonal Mouse IgG    _2A Clone # 797837

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Phosphopeptide containing the human BTK Y551 site

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  See below

• 
  

• Simple Western

  20 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BTK

Bruton tyrosine kinase (Btk) is a 75 kDa cytoplasmic protein tyrosine kinase that is widely expressed in hematopoietic cells. Btk is required for B cell receptor signaling and B cell development. Defects in Btk result in X-linked agammaglobulinemia which is characterized by a severely decreased level of circulating antibodies. Like other Tec family kinases, Btk contains a Pleckstrin homology domain, a Tec homology domain, an SH3 domain, an SH2 domain, and a protein kinase domain. Association of the Btk SH2 domain with the B cell linker protein (BLNK) is required for the activation of PLCg by Btk. Btk can be activated by Lyn or Syk phosphorylation of Tyr551 within the catalytic domain. Within aa 546 - 556, human Btk shares 100% aa sequence identity with mouse and rat Btk.

• Long Name:

  Bruton Agammaglobulinemia Tyrosine Kinase

• Entrez Gene IDs:

  695 (Human)

• Alternate Names:

  Agammaglobulinaemia tyrosine kinase; AGMX1; AGMX1MGC126262; AT; ATKIMD1; B-cell progenitor kinase; BPK; Bruton agammaglobulinemia tyrosine kinase; Bruton tyrosine kinase; BTK; dominant-negative kinase-deficient Brutons tyrosine kinase; EC 2.7.10; EC 2.7.10.2; IMD1; PSCTK1; tyrosine-protein kinase BTK; XLA; XLAMGC126261