• Species Reactivity

  Human

• Specificity

  Detects human BMP-2 and human BMP-4 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant zebra fish (rzf) BMP-2 and rhBMP-4, 20%-40% cross-reactivity with rhBMP-3, -3b, -6, and no cross-reactivity with rhBMP-5, -7, -10, or -15 is observed.    
   

• Source

  Monoclonal Mouse IgG    ₁ Clone # 100230

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant human BMP-2    
  Gln283-Arg396    
  Accession # P12643

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  Recombinant Human BMP-2 (Catalog # )    
  Recombinant Human BMP-4 (Catalog # )    
  under non-reducing conditions only

• 
  

• Neutralization

  Measured by its ability to neutralize BMP?2-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. Nakamura, K.     et al. (1999) Exp. Cell Res.     250:351. The Neutralization Dose (ND    ₅₀) is typically 5-15 μg/mL in the presence of 1 μg/mL Recombinant Human BMP?2 and 2 μg/mL heparin. This antibody also neutralizes Human BMP-4 induced activity in the ATDC5 mouse chondrogenic cell line. The ND    ₅₀ is approximately 3 μg/mL in the presence of 75 ng/mL of Recombinant Human BMP-4.

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-2/BMP-4

Bone Morphogenetic Protein 2 (BMP-2) and BMP-4 are two of at least 15 structurally and functionally related BMPs, which are members of the transforming growth factor  beta (TGF-beta ) superfamily, BMPs were originally identified as protein regulators of cartilage and bone formation. However, they have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs.

• Long Name:

  Bone Morphogenetic Protein 2/4

• Entrez Gene IDs:

  650 (Human); 12156 (Mouse); 29373 (Rat)

• Alternate Names:

  BDA2; BMP-2; BMP-2/BMP-4; BMP2A; BMP-2A; BMP2ABone morphogenetic protein 2A; bone morphogenetic protein 2