• Species Reactivity

  Human

• Specificity

  Detects human ASGR/1ASGPR1 in direct ELISAs.    
   

• Source

  Monoclonal Mouse IgG    ₁ Clone # 950216

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human ASGR1/ASGPR1    
  Gln62-Leu291    
  Accession # P07306

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25 μg/10    ⁶ cells

  See below

• 
  

• Immunocytochemistry

  5-25 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ASGR1/ASGPR1

The human asialoglycoprotein receptor (ASGPR) is an endocytic recycling receptor that belongs to the long-form subfamily of the C-type/Ca⁺²-dependent lectin family (1, 2, 3). It is a complex of two noncovalently-linked subunits, a major 46 kDa glycoprotein (ASGR1) and a minor 50 kDa glycoprotein (ASGR2). The major human ASGPR subunit, ASGR1 (also H1), is synthesized as a 291 amino acid (aa) type II transmembrane (TM) glycoprotein. It contains a 40 aa cytoplasmic region, a 21 aa TM segment, and a 230 aa extracellular domain (ECD) (4 - 6). The cytoplasmic region contains one palmitoylation site at Cys36 that is essential for ligand endocytosis and dissociation (7). The ECD contains two important structural regions. The first is a stalk region of 62 aa (aa 61 - 123) that contributes to noncovalent oligomerization. The second is a 118 aa, carbohydrate-binding, Ca⁺²-dependent C-type lectin domain (aa 161 - 278) that is stabilized by three Ca⁺² ions (3, 8). Human ASGR1 ECD is 79% aa identical to mouse ASGR1 ECD. There are two minor (ASGR2) subunits that interact with ASGR1/H1 in a mutually exclusive manner to generate a functional ASGPR (9). They represent alternate splice forms of a type II TM protein. Termed H2b and H2c, H2b differs from H2c only by the presence of a 19 aa insert in its cytoplasmic region. This insert is significant because it allows serine phosphorylation of the cytoplasmic tail and provides for the majority of ASGPR ligand internalization (9). The stoichiometry of a functional ASGPR is unclear, but is suggested to be either a 2:2, 3:1 or 3:2 ratio of ASGR1/H1:ASGR2/H2 (9, 10, 11). ASGPR is found on hepatocytes and a subset of T cells (6, 12). ASGPR is reported to bind Gal (nonreducing), GalNAc, and sialic acid alpha 2,6Gal and GalNAc (3, 13, 14, 15). This is generally within the context of triantennary or tetraantennary configurations (2). The sialic acid terminations are of particular interest because molecules with these motifs most likely represent the endogenous ligands for ASGPR (14).

• References:

1. Stockert, R. J. (1995) Physiol. Rev. 75:591.

2. Weigel, P.H. and J.H.N. Yik (2002) Biochim. Biophys. Acta 1572:341.

3. Meier, M. et al. (2000) J. Mol. Biol. 300:857.

4. Spiess, M. et al. (1985) J. Biol. Chem. 260:1979.

5. Spiess, M. and H.F. Lodish (1986) Cell 44:177.

6. Bischoff, J. et al. (1988) J. Cell Biol. 106:1067.

7. Yik, J.H.N. et al. (2002) J. Biol. Chem. 277:40844.

8. Monroe, R.S. and B.E. Huber (1994) Gene 148:237.

9. Yik, J.H.N. et al. (2002) J. Biol. Chem. 277:23076.

10. Bider, M.D. et al. (1996) J. Biol. Chem. 271:31996.

11. Lodish, H. (1991) Trends Biochem. Sci. 16:374.

12. Park, J-H. et al. (2006) Biotechnol. Lett. 28:1061.

13. Westerlind, U. et al. (2004) Glyconj. J. 21:227.

14. Park, E.I. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17125.

15. Park, E.I. et al. (2003) J. Biol. Chem. 278:4597.

• Long Name:

  Asialoglycoprotein Receptor 1

• Entrez Gene IDs:

  432 (Human); 11889 (Mouse); 24210 (Rat)

• Alternate Names:

  ASGPR 1; ASGP-R 1; ASGPR; ASGPR1; ASGR1; asialoglycoprotein receptor 1; CLEC4H1; CLEC4H1ASGPR1; C-type lectin domain family 4 member H1; Hepatic lectin H1; HL-1; MHL1; RHL1