• Species Reactivity

  Human

• Specificity

  Detects human GIPR in direct ELISAs. Stains human GIPR transfected cells but not irrelevant transfectants.    
   

• Source

  Monoclonal Mouse IgG1 Clone # 591853

• Immunogen

  NS0 mouse myeloma cell line transfected with human GIPR.    
  Met1-Cys466    
  Accession # P48546

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 350

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 μg/10    ⁶ cells

  HEK293 human embryonic kidney cell line transfected with human GIPR and eGFP

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GIPR

GIPR is a 7-transmembrane receptor for GIP (glucose-dependent insulinotropic polypeptide or gastric inhibitory polypeptide). The 466 amino acid (aa) human GIPR contains 176 extracellular domain (ECD) aa that share 77% and 81% aa identity with mouse and rat GIPR ECD, respectively. A splice isoform of 430 aa has a deletion of aa 58-93 in the N-terminal ECD, while isoforms of 491 and 419 aa have alternate C-terminal cytoplasmic sequences. Engagement by GIP on pancreatic b?cells activates adenylate cyclase to regulate insulin compensation in the presence of high circulating glucose. GIPR is also expressed on adipocytes, osteoblasts and myelinating Schwann cell membranes.

• Long Name:

  Gastric Inhibitory Polypeptide Receptor

• Entrez Gene IDs:

  2696 (Human); 381853 (Mouse); 25024 (Rat)

• Alternate Names:

  gastric inhibitory polypeptide receptor; GIPR; GIP-R; Glucose-dependent insulinotropic polypeptide receptor; MGC126722; PGQTL2