• Species Reactivity

  Mouse

• Specificity

  Detects mouse CD96 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CD96v2 is observed.

• Source

  Monoclonal Rat IgG    _2A Clone # 630612

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse CD96    
  Val22-Met536    
  Accession # Q3U0X8

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 μL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD96

Mouse CD96, also known as Tactile, is a 170-180 kDa member of the Ig-Superfamily.  It is expressed on CD4⁺ and CD8⁺ T cells, NK and NKT cells, resting monocytes and gamma δ T cells.  Mouse CD96 binds to CD155 and Nectin-1, and likely participates in cell-to-cell adhesion.  Mature mouse CD96 is a 581 amino acid (aa), type I transmembrane glycoprotein.  It contains a 515 aa extracellular region (aa 22-536) that contains three Ig-like domains, plus a 45 aa cytoplasmic region.  The two N-terminal domains are V-type (aa 24-244), while the distal domain is a C-type structure (aa 250-355).  Unlike human, there is no splice variant in the second V-type domain.  There is, however, a potential isoform that shows a single Cys substitution for aa 437-602.  Over aa 1-536, mouse CD96 shares 55% and 79% aa identity with human and rat CD96, respectively.

• Entrez Gene IDs:

  10225 (Human); 84544 (Mouse); 498079 (Rat)

• Alternate Names:

  CD96 antigenMGC22596; CD96 molecule; CD96; DKFZp667E2122; T cell-activated increased late expression protein; TACTILE; TACTILEincreased late expression; T-cell surface protein tactile