• Species Reactivity

  Human

• Specificity

  Detects human MSX2 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human MSX1 is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 786607

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human MSX2    
  Ala2-Thr132    
  Accession # P35548

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Immunocytochemistry

  8-25 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MSX2

MSX2, also known as HOX-8, is an approximately 35 kDa homeobox transcription factor that is a downstream target of canonical Wnt signaling. MSX2 plays an important role in the development of mineralized tissues including bone, cartilage, and teeth. It is upregulated in atherosclerotic plaques and promotes the calcification of vascular smooth muscle cells during inflammation. MSX2 suppresses the adipocytic differentiation of multipotent mesenchymal cells. MSX2 acts in mammary gland development and the initiation of female fetal germ cell meiosis. It promotes tumorigenesis of breast and pancreatic carcinomas but inhibits the survival and invasiveness of melanoma cells. Human MSX2 contains one homeodomain (aa 143-201). Within aa 1-132, human MSX2 shares 87% aa sequence identity with mouse and rat MSX2.

• Long Name:

  Msh Homeobox 2

• Entrez Gene IDs:

  4488 (Human); 17702 (Mouse); 25483 (Rat)

• Alternate Names:

  CRS2; CRS2parietal foramina 1; FPP; Homeobox protein Hox-8; homeobox protein MSX-2; Hox8; HOX8msh homeobox homolog 2 (Drosophila); msh (Drosophila) homeo box homolog 2; msh homeo box 2; msh homeobox 2; msh homeobox homolog 2; MSH; MSX2; PFM; PFM1