• Species Reactivity

  Human

• Specificity

  Detects human PDZD2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse PDZD2 is observed.

• Source

  Monoclonal Mouse IgG    _2A Clone # 787924

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human PDZD2    
  Ser2489-Ser2839    
  Accession # O15018

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  2 μg/mL

  See below

• 
  

• Immunocytochemistry

  8-25 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDZD2

PDZD2 (PSD95, Discs-large and ZO-1 domain-containing protein 2), also called AIPC (activated in prostate cancer) and PDZK3, is a multi-PDZ-domain protein that is ubiquitously expressed. The 2839 amino acid (aa) human PDZD2 is found in the endoplasmic reticulum. Caspase cleavage produces the 347 aa, 37 kDa secreted mature form (aa 2493-2839). A 2640 aa isoform is lacking 199 aa N-terminal to the mature sequence. The processed, mature form contains two PDZ domains which interact with the C-terminus of partner proteins, promotes maturation of pancreatic b cells, and is highly expressed in human prostate tumors. Human PDZD2 shares 72% aa sequence identity with mouse or rat PDZD2.

• Long Name:

  PDZ Domain Containing 2

• Entrez Gene IDs:

  23037 (Human); 68070 (Mouse); 65034 (Rat)

• Alternate Names:

  Activated in prostate cancer protein; AIPC; AIPCPAPIN; KIAA0300; KIAA0300PDZ domain-containing protein 3; Papin; PDZ domain containing 2; PDZ domain containing 3; PDZ domain-containing protein 2; PDZD2; PDZK3; Pin1