• Species Reactivity

  Mouse

• Specificity

  Detects mouse IFN? beta in direct ELISAs.    
   

• Source

  Recombinant Monoclonal Rabbit IgG Clone # 1176D

• Purification

  Protein A or G purified from cell culture supernatant

• Immunogen

  Human embryonic kidney cell line HEK293-derived mouse IFN? beta    
  Ile22-Asn182    
  Accession # P01575

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Neutralization

  Measured by its ability to neutralize IFN? beta inhibition of viral-induced cytopathy in the L?929 mouse fibroblast cell line. The Neutralization Dose (ND    ₅₀) is typically 0.25-1.25 μg/mL in the presence of 10 pg/mL Recombinant Mouse IFN? beta.

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-beta

Interferon beta (IFN-beta ), also known as fibroblast IFN, is a secreted, approximately 22 kDa member of the type I interferon family of molecules (1). Mature mouse IFN-beta shares 75% and 47% amino acid sequence identity with the rat and human proteins, respectively. Fibroblasts are the major producers of IFN-beta, but it can also be produced by dendritic cells, macrophages, and endothelial cells in response to pathogens (2). It is transcriptionally regulated by TRAF3, IRF3, IRF7, and NF-kappa B (3, 4). IFN-beta -deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS) (5). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS (6-10). IFN-beta can additionally induce the expression of the anti-inflammatory cytokine IL-10 (11).

• References:

1. González-Navajas, J.M. et al. (2012) Nat. Rev. Immunol. 12:125.

2. Reder, A.T. and X. Feng (2013) Front. Immunol. 4:281.

3. Schafer, S.L. et al. (1998) J. Biol. Chem. 273:2714.

4. H?cker, H. et al. (2006) Nature 439:204.

5. Teige, I. et al. (2003) J. Immunol. 170:4776.

6. Shinohara, M.L. et al. (2008) Immunity 29:68.

7. Guo, B. et al. (2008) J. Clin. Invest. 118:1680.

8. Ramgolam, V.S. and S. Markovic-Plese (2010) Endocr. Metab. Immune Disord. Drug Targets 10:161.

9. Martín-Saavedra, F.M. et al. (2008) Mol. Immunol. 45:4008.

10. Inoue, M. and M.L. Shinohara (2013) Immunology 139:11.

11. Wang, H. et al. (2011) J. Immunol 186:675.

• Long Name:

  Interferon beta

• Entrez Gene IDs:

  3456 (Human); 15977 (Mouse); 24481 (Rat)

• Alternate Names:

  Fibroblast interferon; IFBIFNB; IFF; IFNB1; IFNbeta; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956