• Species Reactivity

  Human, Mouse, Rat

• Specificity

  Detects human, mouse, and rat ATG5 in Western blots.

• Source

  Monoclonal Mouse IgG    _2B Clone # 603813

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human ATG5    
  Asn99-Thr193    
  Accession # Q9H1Y0

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 594

Applications

• Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  5 μL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ATG5

ATG5 (Autophagy-related Protein), also known as APG5L and Apoptosis-specific Protein, is a ubiquitous 32 kDa member of the ATG family of proteins. ATG5 exists as a covalent heterodimer with ATG12 through the creation of a Lys-Gly linkage. The ATG5:ATG12 heterodimer associates noncovalently with an ATG16 multimer to generate autophagosomes. Human ATG5 is 275 amino acids in length and contains N- and C-terminal ubiquitin-like domains (aa 15?105 and 187?275) separated by a helix-rich linker region that contains a dimerizing Lys at position 130. There are two potential alternate start sites at Met80 and Met173. Over aa 99?193, human ATG5 is 97% aa identical to mouse ATG5.

• Long Name:

  ATG5 Autophagy Related 5 Homolog

• Entrez Gene IDs:

  9474 (Human); 11793 (Mouse)

• Alternate Names:

  APG5 autophagy 5-like (S. cerevisiae); APG5; APG5LS. cerevisiae)-like; ASP; ASPAPG5-LIKE; ATG5 autophagy related 5 homolog (S. cerevisiae); ATG5; Autophagy protein 5