• Species Reactivity

  Mouse

• Specificity

  Detects mouse IGF-I in direct ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant human (rh) IGF?I, rmIGF-II, rmIGFBP-2, rmIGFBP-5, and rmIGFBP-6.

• Source

  Monoclonal Hamster IgG Clone # 126002

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant mouse IGF-I    
  Gly49-Ala118    
  Accession # P05017

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  Recombinant Mouse IGF-I (Catalog # )

• 
  

• Mouse IGF-I Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  2-8 μg/mL 

  Mouse IGF?I Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.1-0.4 μg/mL 

  Mouse IGF?I Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Mouse IGF-I Protein, CF (Catalog # )

• 
  

• Neutralization

  Measured by its ability to neutralize IGF?I-induced proliferation in the MCF?7 human breast cancer cell line. Karey, K.P.      et al. (1988) Cancer Research      48:4083. The Neutralization Dose (ND     ₅₀) is typically 0.3-1.5 μg/mL in the presence of 15 ng/mL Recombinant Mouse IGF?I.

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGF-I

Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Mouse IGF-I is synthesized as two precursor isoforms with alternate N- and C-terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I is identical between isoforms and is generated by proteolytic removal of the N- and C-terminal regions. Mature mouse IGF-I shares 94% and 99% amino acid (aa) sequence identity with human and rat IGF-I, respectively (2), and exhibits cross-species activity. It shares 60% aa sequence identity with mature mouse IGF-II. Circulating IGF-I is produced by hepatocytes, while local IGF-I is produced by many other tissues in which it has paracrine effects (1). IGF-I induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (3). IGF-I regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (4-7). It plays an important role in muscle regeneration and tumor progression (1, 8). IGF-I binds IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (9). IGF-I association with IGF binding proteins increases its plasma half-life and modulates its interactions with receptors (10).

• References:

1. Philippou, A. et al. (2007) In Vivo 21:45.

2. Bell, G.I. et al. (1986) Nucleic Acids Res. 14:7873.

3. Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890.

4. Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620.

5. Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577.

6. Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275.

7. Malemud, C.J. (2007) Clin. Chim. Acta 375:10.

8. Samani, A.A. et al. (2007) Endocrine Rev. 28:20.

9. LeRoith, D. and S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302.

10. Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.

• Long Name:

  Insulin-like Growth Factor I

• Entrez Gene IDs:

  3479 (Human); 16000 (Mouse); 24482 (Rat)

• Alternate Names:

  IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin A; Somatomedin C; somatomedin-C