詳細(xì)說(shuō)明
Species Reactivity
Human
Specificity
Detects human T?bet/TBX21 in direct ELISAs.
Source
Monoclonal Mouse IgG 1 Clone # 525831
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human T?bet/TBX21
Glu326-Asn535
Accession # Q9UL17Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 750
Applications
Recommended
ConcentrationSample
Intracellular Staining by Flow Cytometry
0.25-1 μg/10 6 cells
Human peripheral blood mononuclear cells (PBMCs) treated with Anti-Human IL-4 Polyclonal Antibody (Catalog # AB-204-NA; 20 μg/mL) and Recombinant Human IL?12 (Catalog # ;10 ng/mL) for 5 days to induce Th1 cell development were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # )
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: T-bet/TBX21
T-box expressed in T cells (T-bet), also known as T-box transcription factor TBX21, is a 62 kDa member of the T-box family of transcription factors and the Tbr1 subfamily. Human T-bet is 535 amino acids in length and contains a T-box DNA binding domain (aa 136-327). Human T-bet shares 88% aa sequence identity with mouse T-bet. T-bet is a nuclear protein highly apparent in Th1 cells. Northern blot analysis revealed that it is also expressed in lung, thymus and spleen. Functionally, T-bet controls the expression of the Th1 cytokine, IFN-gamma, and initiates Th1 lineage development from na?ve Th precursor cells by both activating Th1 genetic programs and by repressing the opposing Th2 programs.
Long Name:
T-box transcription factor TBX21
Entrez Gene IDs:
30009 (Human); 57765 (Mouse); 303496 (Rat)
Alternate Names:
Tbet; T-bet; TBETT-PET; TBLYM; TBLYMT-cell-specific T-box transcription factor T-bet; T-box 21; T-box expressed in T cells; T-box protein 21; T-box transcription factor TBX21; TBX21; T-PET; Transcription factor TBLYM