• Species Reactivity

  Mouse

• Specificity

  Detects mouse DMBT1 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) CD163 and rhSRCRB4D is observed.

• Source

  Monoclonal Rat IgG2b Clone # 548031

• Immunogen

  E. Coli-derived recombinant mouse DMBT1    
  Trp215-Gly420    
  Accession # Q60997

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 350

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 μg/10    ⁶ cells

  CMT?93 mouse rectal carcinoma cell line

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: DMBT1

DMBT1 (deleted in malignant brain tumors 1), also called salivary agglutinin (SAG) in humans, hensin in rabbit, ebnerin in rat, and muclin, crp-ductin or vomeroglandin in mouse, is a transmembrane or secreted ~340 kDa, 2085 amino acid (aa) protein of the scavenger receptor cysteine-rich (SRCR) family. It contains several SRCR and CUB domains and a zona pellucida motif. The region used as the immunogen (aa 215-420) contains most of SRCR 2 and 3, and shares ~74% aa identity with corresponding regions of rat or human DMBT1. Several alternately spliced, glycosylated and sulfated forms exist. DMBT1 functions in mucosal immunity by binding bacterial, viral and endogenous proteins to aid clearance by macrophages. It is also thought to play a role in epithelial cell differentiation and tumor suppression.

• Long Name:

  Deleted in Malignant Brain Tumors 1

• Entrez Gene IDs:

  1755 (Human); 12945 (Mouse); 170568 (Rat)

• Alternate Names:

  CRP-ductin; deleted in malignant brain tumors 1 protein; deleted in malignant brain tumors 1; DMBT1; Ebnerin; FLJ61058; Glycoprotein 340; GP340; gp-340; GP340muclin; Hensin; MGC164738; Muclin; SAG; Salivary agglutinin; Surfactant pulmonary-associated D-binding protein; Vomeroglandin