• Species Reactivity

  Mouse

• Specificity

  Detects mouse ST2/IL-33 R in ELISAs.

• Source

  Monoclonal Rat IgG2b Clone # 245707

• Immunogen

  S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse ST2/IL-33 R    
  Ser27-Arg332    
  Accession # P14719

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 μg/10    ⁶ cells

  P815 mouse mastocytoma cell line

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ST2/IL-33 R

ST2, also known as IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses (1, 2). Mouse ST2 consists of a 306 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 23 aa transmembrane segment, and a 212 aa cytoplasmic domain with an intracellular TIR domain (3). Alternate splicing of the 120 kDa mouse ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions (3). Within the ECD, mouse ST2 shares 68% and 81% aa sequence identity with human and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (4?7). It binds IL-33, a cytokine that is up?regulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (4, 8). IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL-1 R rp2 (1, 9, 10). In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (6). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (4, 11?13). The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (5, 7, 14). Soluble ST2 functions as a decoy receptor that blocks IL-33’s ability to signal through transmembrane ST2 (9, 12?14).

• References:

1. Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.

2. Gadina, M. and C.A. Jefferies (2007) Science STKE 2007:pe31.

3. Yanagisawa, K. et al. (1993) FEBS Lett. 318:83.

4. Schmitz, J. et al. (2005) Immunity 23:479.

5. Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979.

6. Brint, E.K. et al. (2004) Nat. Immunol. 5:373.

7. Weinberg, E.O. et al. (2002) Circulation 106:2961.

8. Sanada S. et al. (2007) J. Clin. Invest. 117:1538

9. Palmer, G. et al. (2008) Cytokine 42:358.

10. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.

11. Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051.

12. Verri Jr., W.A. et al. (2008) Proc. Natl. Acad. Sci. 105:2723.

13. Miller, A.M. et al. (2008) J. Exp. Med. 205:339.

14. Hayakawa, H. et al. (2007) J. Biol. Chem. 282:26369.

• Long Name:

  Interleukin 33 Receptor

• Entrez Gene IDs:

  9173 (Human); 17082 (Mouse)

• Alternate Names:

  DER4; Fit-1; IL-1 R4; IL1R4; IL-1R4; IL-1RL1; IL-33R; Ly84; ST2; ST2L; ST2V; suppression of tumorigenicity 2; T1