• Purity

  >85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of BUD?8 human fibroblast cells. When 3 x 10    ⁴ cells/well are added to Recombinant Human Desmocollin-1 coated plates (10 μg/mL, 100 μL/well), >45% will adhere after 90 minutes at 37 °C.

• Source

  Mouse myeloma cell line, NS0-derived Arg135-Asn686, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Arg135

• Predicted Molecular Mass

  63 kDa

• SDS-PAGE

  80-85 kDa, reducing conditions

Background: Desmocollin-1

Desmocollin-1 (DSC1) is a 100 kDa-110 kDa transmembrane glycoprotein in the cadherin family of calcium dependent adhesion molecules (1-3). Human DSC1 is synthesized with a 105 amino acid (aa) propeptide. The mature protein consists of a 557 aa extracellular domain (ECD) that contains five cadherin-like domains, a 23 aa transmembrane segment, and a 180 aa cytoplasmic domain (4, 5). Within the ECD, human DSC1 shares 79% and 82% aa sequence identity with bovine and mouse DSC1, respectively, and 53% with human DSC2 and DSC3. An alternately spliced isoform has a substituted and truncated cytoplasmic domain (5). DSC1 is one of the principal components of desmosomes which form adhesive contacts between epithelial cells (1, 2). It is predominantly expressed in the outer layers of stratified epithelia of the skin, tongue, and hair follicle root sheath (6, 7). It is required for both promoting epidermal differentiation and maintaining epidermal strength (8). DSC2 and DSC3, by contrast, are preferentially localized in the basal and suprabasal layers of the epidermis (1). DSC1 is not expressed in normal colon epithelium, but it is induced during colon carcinogenesis (9). Loss of DSC1 function in the skin disorders IgA pemphigus and Netherton Syndrome results from autoimmune targeting or enhanced KLK-7 mediated proteolysis, respectively (10-12).

• References:

1. Kottke, M.D. et al. (2006) J. Cell Sci. 119:797.

2. Garrod, D.R. et al. (2002) Mol. Membrane Biol. 19:81.

3. Leckband, D. and A. Prakasam (2006) Annu. Rev. Biomed. Eng. 8:259.

4. King, I.A. et al. (1993) Genomics 18:185.

5. Theis, D.G. et al. (1993) Int. J. Dev. Biol. 37:101.

6. King, I.A. et al. (1996) J. Invest. Dermatol. 107:531.

7. Nuber, U.A. et al. (1996) Eur. J. Cell Biol. 71:1.

8. Chidgey, M. et al. (2001) J. Cell Biol. 155:821.

9. Khan, K. et al. (2006) Br. J. Cancer 95:1367.

10. Hashimoto, T. et al. (1997) J. Invest. Dermatol. 109:127.

11. Caubet, C. et al. (2004) J. Invest. Dermatol. 122:1235.

12. Descargues, P. et al. (2006) J. Invest. Dermatol. 126:1622.

• Entrez Gene IDs:

  1823 (Human); 13505 (Mouse); 291759 (Rat)

• Alternate Names:

  cadherin family member 1; CDHF1; desmocollin 1; Desmocollin1; Desmocollin-1; desmosomal glycoprotein 2/3; DG2/DG3 ; DG2/DG3; DSC1