• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.01 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to bind alpha -L-Fucose. Keshi, H.     et al. (2006) Microbiol. Immunol.     50(12):1001.

• Source

  Mouse myeloma cell line, NS0-derived Gln26-Met271, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  No results obtained: Gln26 predicted

• Predicted Molecular Mass

  27 kDa

• SDS-PAGE

  36 kDa, reducing conditions

Background: CL-K1/COLEC11

Collectins constitute a family of C-type lectins that recognize molecular patterns expressed on pathogens. Members of this glycoprotein family contain an N-terminal domain, a collagen-like domain, a neck region, and a C-terminal carbohydrate recognition domain (CRD). Collectins are typically secreted molecules, although CL-P1 is membrane bound and CL-L1 is found in the cytoplasm (1 - 3). Collectin kidney 1 (CL-K1), also known as collectin subfamily member 11 (COLEC11), is a 37 kDa collectin that circulates in the blood (4, 5). Alternative splicing may generate multiple isoforms with deletions or substitutions in the N-terminal and collagen-like domains (6). CL-K1 is widely expressed, notably in renal proximal tubules, bronchial glands, and gastrointestinal mucosa (5). It associates into disulfide-linked oligomers and preferentially interacts with fucose residues in a calcium-dependent manner (4). Mature human CL-K1 shares 93% amino acid sequence identity with mouse and rat CL-K1. Within the CRD, human CL-K1 shares 55% aa sequence identity with CL-L1 and 26% - 33% aa sequence identity with collectins CL-P1, MBL, SP-A, and SP-D.

• References:

1. Gupta, G. and A. Surolia, 2007, Bioessays 29:452.

2. van de Wetering, J.K. et al., 2004, Eur. J. Biochem. 271:1229.

3. Holmskov, U. et al., 2003, Annu. Rev. Immunol. 21:547.

4. Keshi, H. et al., 2006, Microbiol. Immunol. 50:1001.

5. Motomura, W. et al., 2008, J. Histochem. Cytochem. 56:243.

6. SwissProt # Q9BWP8.

   
   

• Long Name:

  Collectin Kidney 1

• Entrez Gene IDs:

  78989 (Human); 71693 (Mouse)

• Alternate Names:

  CDC28/CDC2-like kinase; CDC-like kinase 1CLKEC 2.7.12.1; CLK/STY; CLK1; CL-K1; COLEC11; dual specificity protein kinase CLK1; protein tyrosine kinase STY; STY