• Purity

  >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to promote the expansion of E16 rat liver mononuclear cells     in vitro, in the presence of Recombinant Mouse SCF/c?kit Ligand (Catalog # ), Recombinant Mouse Thrombopoietin/Tpo (Catalog # ), and Recombinant Mouse Flt?3 Ligand (Catalog # ). The ED    ₅₀ for this effect is 0.12-0.6 μg/mL in the presence of cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # ).

• Source

  Mouse myeloma cell line, NS0-derived Gly19-Phe323, with an N-terminal Met and a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Met

• Predicted Molecular Mass

  34.7 kDa

• SDS-PAGE

  50 kDa, reducing conditions

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: SCGF/CLEC11a

Stem Cell Growth Factor (SCGF), also known as LSLCL, P47, and CLEC11A, is a secreted glycoprotein that acts on primitive hematopoietic stem/progenitor cells to support their proliferation and differentiation. Mature human SCGF contains an N?terminal Glu?rich region with an RGD motif, a leucine zipper region, and a C?terminal lectin?like domain (1, 2). The RGD motif is absent in mouse and rat SCGF (1). Full length human SCGF (the alpha isoform) shares approximately 83% amino acid (aa) sequence identity with mouse and rat SCGF. A shorter SCGF beta isoform lacks 78 aa of the lectin domain (1?3). This recombinant protein corresponds to the SCGF alpha isoform. SCGF is produced by various hematopoietic cell lines and by CD34⁺ and CD34?CD33⁺ bone marrow cells as a 47 kDa sulfated and O?glycosylated sialoprotein (2?6). It is also expressed by infiltrating macrophages in some gastrointestinal tract tumors (7). Mouse SCGF mRNA has been detected in proliferating chondrocytes, the primary ossification center, perichondrium, and periosteum (6). SCGF should not be confused with the similarly named SCF (stem cell factor)/c?Kit Ligand which exerts distinct activities during hematopoietic differentiation. SCGF exhibits erythroid burst (BFU?E) promoting activity in the absence of SCF/c?kit Ligand but suppresses the formation of erythroid bursts induced by SCF (4, 6). SCGF cooperates with IL?3, Flt?3 Ligand, GM?CSF, or G?CSF to enhance the growth of granulocyte?macrophage colonies (CFU?GM) (4, 6). It also promotes (with Flt?3 Ligand and VEGF) the maintenance and expansion of CD34⁺CD133⁺ progenitor cells as well as their differentiation into the endothelial lineage (8, 9). Serum levels of SCGF increase during stem cell transplantation and correlate with the recovery of hematopoiesis (5). Circulating SCGF levels are decreased in severe malarial anemia, and an SCGF promoter polymorphism (-539C/T) is associated with resistance to malarial anemia (10, 11).

• References:

1. Mio, H. et al. (1998) Biochem. Biophys. Res. Commun. 249:124.

2. Bannwarth, S. et al. (1998) J. Biol. Chem. 273:1911.

3. Hiraoka, A. et al. (1997) Proc. Natl. Acad. Sci. 94:7577.

4. Hiraoka, A. et al. (1987) Cancer Res. 47:5025.

5. Ito, C. et al. (2003) Bone Marrow Transplant. 32:391.

6. Hiraoka, A. et al. (2001) Hematol. J. 2:307.

7. Da Riva, L. et al. (2011) J. Transl. Med. 9:158.

8. Gehling, U.M. et al. (2000) Blood 95:3106.

9. Loges, S. et al. (2004) Stem Cells Dev. 13:229.

10. Keller, C.C. et al. (2009) Infect. Immun. 77:3864.

11. Ouma, C. et al. (2010) Infect. Immun. 78:453.

• Long Name:

  Stem Cell Growth Factor/C-type Lectin Domain Family 11, Member A

• Entrez Gene IDs:

  6320 (Human); 20256 (Mouse); 29313 (Rat)

• Alternate Names:

  CLEC11a; CLECSF3; LSLCL; SCGF