• Purity

  >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured in a cytotoxicity assay using HT?29 human colon adenocarcinoma cells Yu, K.Y.     et al. (1999) J. Biol. Chem.     274:13733; Harrop, J.A.     et al. (1998) J. Biol. Chem.     273:27548; Zhai, Y.     et al. (1998) J. Clin. Invest.     102:1142. Recombinant Human LIGHT/TNFSF14 at 10 ng/mL causes a 2-4.5 fold inhibition of HT29 proliferation, in the presence of 10 U/mL (1 ng/mL) Recombinant Human IFN-gamma (Catalog # ). Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G.     et al. (1990) Proc. Natl. Acad. Sci. USA     87:1323. The ED    ₅₀ for this effect is 1-4 ng/mL.

• Source

  Mouse myeloma cell line, NS0-derived

  Met	10-His tag	GGGSGGGSGGGSIEGR	Human Light (Asp74-Val240) Accession # O43557
  N-terminus			C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Met

• Predicted Molecular Mass

  20.9 kDa

• SDS-PAGE

  25 kDa, reducing conditions

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Data Images

Background: LIGHT/TNFSF14

Human LIGHT is a type II membrane protein that is a member of the TNF superfamily. LIGHT is an acronym which stands for "is homologous to lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes". LIGHT has also been called HVEM-L and LT-gamma. Under the new TNF nomenclature, it is called TNFSF14. LIGHT is a 240 amino acid (aa) protein that contains a 37 aa cytoplasmic domain, a 22 aa transmembrane region, and a 181 aa extracellular domain. Similar to other TNF ligand family members, LIGHT is predicted to assemble as a homotrimer. LIGHT is produced by activated T cells and was first identified by its ability to compete with HSV glycoprotein D for HVEM binding. LIGHT has also been shown to bind to the lymphotoxin beta receptor (LT beta R) and the decoy receptor (DcR3/TR6). LIGHT overexpression in tumor cells induces apoptosis, which can be enhanced by IFN-gamma. The full roles of LIGHT remain to be elucidated.

• References:

1. Mauri, D.N. et al. (1998) Immunity 8:21.

2. Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.

3. Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548.

4. Yu, K-Y. et al. (1999) J. Biol. Chem. 274:13733.

• Long Name:

  TNF Ligand Superfamily Member 14

• Entrez Gene IDs:

  8740 (Human); 50930 (Mouse)

• Alternate Names:

  CD258 antigen; CD258; delta transmembrane LIGHT; Herpes virus entry mediator ligand; Herpesvirus entry mediator ligand; HVEM-L; HVEMLherpesvirus entry mediator-ligand; ligand for herpesvirus entry mediator; LIGHT; LIGHTherpesvirus entry mediator A; LTg; TNFSF14; TR2; tumor necrosis factor (ligand) superfamily, member 14; tumor necrosis factor ligand superfamily member 14; tumor necrosis factor receptor-like 2; tumor necrosis factor superfamily member LIGHT