詳細(xì)說(shuō)明
Species Reactivity
Human
Specificity
Detects human Pro?Corticoliberin/Pro?CRH in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Pro?Corticoliberin/Pro?CRH
Leu25-Lys196
Accession # P06850Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.
Label
Unconjugated
Applications
Recommended
ConcentrationSample
Western Blot
1 μg/mL
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Western Blot | Detection of Human Pro-Corticoliberin/Pro-CRH by Western Blot. Western blot shows lysates of human cerebellum and hypothalamus tissue. PVDF membrane was probed with 1 μg/mL of Sheep Anti-Human Pro?Corticoliberin/Pro?CRH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5805) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # ). A specific band was detected for Pro?Corticoliberin/Pro?CRH at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using . |
Preparation and Storage
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Pro-Corticoliberin/Pro-CRH
Long Name:
Pro-Corticotropin-releasing Hormone
Entrez Gene IDs:
1392 (Human); 12918 (Mouse); 81648 (Rat)
Alternate Names:
CRF; CRH; ProCorticoliberin; Pro-Corticoliberin