• Species Reactivity

  Human

• Specificity

  Detects human Integrin alpha V beta 3.

• Source

  Monoclonal Mouse IgG1 Clone # 23C6

• Immunogen

  Human osteoclasts

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 594

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 μg/10    ⁶ cells

  HUVEC human umbilical vein endothelial cells

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Integrin alpha V beta 3

Integrin alpha V beta 3 together with alpha IIb beta ₃, constitutes the only known beta 3 Integrins (1?3). The non-covalent heterodimer of 170 kDa alpha V/CD51 and 93 kDa beta ₃/CD61 subunits shows wide expression, notably by endothelial cells and osteoclasts (2?4). Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. Active cell surface alpha V beta 3 adheres to matrix proteins including vitronectin, fibronectin, fibrinogen and thrombospondin (2, 3). The ligand binding site of alpha V beta 3 is in the N-terminal head region, formed by interaction of the beta 3 vWFA domain with the alpha V beta-propeller structure (4). The alpha V subunit contributes a thigh and a calf region, while the beta 3 subunit contains a PSI domain and four cysteine-rich I-EGF folds. The alpha V subunit domains termed thigh, calf-1 and calf-2 generate a “knee” region that is bent when the alpha V beta 3 is in its constitutively inactive state. Activation, either by “inside out” signaling or by Mg²⁺ or Mn²⁺ binding, extends the Integrin to expose its ligand binding site (1, 4). Two splice variants of beta 3 (b and c) diverge over the last 21 amino acids (aa) and lack cytoplasmic phosphorylation sites (5, 6). Another beta 3 splice variant diverges after the vWFA domain, producing a soluble 60 kDa form in platelets and endothelial cells (7). alpha V beta 3 is essential for the maturation of osteoclasts and their binding and resorption of bone; it also, however, promotes their apoptosis (8, 9). M-CSF R and alpha V beta 3 share signaling pathways during osteoclastogenesis, and deletion of either molecule causes osteopetrosis (8, 9). Also cell entry of several viruses is mediated by alpha V beta 3 (4, 10). The 962 aa human alpha V ECD (11) shares 92?95% aa sequence identity with mouse, rat and cow  alpha V while the 685 aa human beta ₃ ECD (12) shares 95% aa identity with horse and dog, and 89?92% aa identity with mouse, rat and pig beta ₃.

• References:

1. Hynes, R. O. (2002) Cell 110:673.

2. Serini, G. et al. (2006) Exp. Cell Res. 312:651.

3. Ross, F. P. and S. L. Teitelbaum (2005) Immunol. Rev. 208:88.

4. Xiong, J. et al. (2001) Science 294:339.

5. Kumar, C. S. et al. (1987) J. Biol. Chem. 272:16390.

6. vanKuppevelt, H. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5415.

7. Djaffar, I. et al. (1994) Biochem. J. 300:67.

8. McHugh, K. P. et al. (2000) J. Clin. Invest. 105:433.

9. Faccio, R. et al. (2003) J. Clin. Invest. 111:749.

10. Chu, J. J. and M. Ng (2004) J. Biol. Chem. 279:54533.

11. Suzuki, S. et al. (1987) J. Biol. Chem. 262:14060.

12. Fitzgerald, L. A. et al. (1987) J. Biol. Chem. 262:3936.

• Entrez Gene IDs:

  3685 (Human)

• Alternate Names:

  antigen identified by monoclonal L230; CD51 antigen; CD51; Integrin alpha V beta 3; integrin alpha-V; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); MSK8; Vitronectin receptor subunit alpha; VNRADKFZp686A08142