• Species Reactivity

  Human

• Specificity

  Delects human MSP R/Ron in direct ELISAs and Western blots. In these formats, approximately 25% cross?reactivity with recombinant mouse MSP R is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human MSP R/Ron    
  Glu25-Ser956    
  Accession # Q04912

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 μL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MSP R/Ron

RON (Recepteur d'Origine Nantais [Receptor originating (or discovered) in Nantes (France)]; also known as MSP-R, PTK8 and MST1R) is a 180-190 kDa member of the tyrosine kinase family, protein kinase superfamily of molecules. Human mature RON is a type I transmembrane heterodimeric glycoprotein that arises from the proteolytic cleavage of a single chain proform 1376 amino acids (aa) in length. Posttranslational processing generates a 35-40 kDa a-chain (aa 25-304) coupled to a 145-150 kDa b-chain (aa 310-1400). The chains are disulfide-linked, and the transmembrane segment appears over aa 958-978. Multiple cell types are known to express RON, and these include osteoclasts, TrkA+ dorsal root ganglia neurons, macrophages, keratinized and nonkeratinized stratified squamous epithelium, intestinal Paneth cells, renal proximal tubule and mesangial cells, monocytes, neutrophils and mammary epithelium. On the cell surface, RON serves as a receptor for MSP, a 78-80 kDa soluble molecule related to HGF. Ligand binding initiates homodimerization and receptor signaling. In addition, RON is also known to heterodimerize with multiple integral membrane proteins, either individually or as part of a complex. Proteins identified to date include b1 integrins, c-MET/HGFR, E-Cadherin, the bc-chain for IL-3, EPO-R, EGFR, IGF-IR, Plexins B1 and B3, and CD44v6. RON may also be found intracellularly, where it interacts with either the androgen receptor or EGFR in the cytoplasm, or in the nucleus, where it acts as a transcription factor coupled to HIF-1a and regulates c-Jun, Bcl-2 and c-FLIP expression. There are about a dozen alternative splice forms for RON, some soluble, while others are transmembrane, and either constitutively active or biochemically inert. They hold the potential to interact with full-length RON and impact its signaling capabilities. Reflecting the diverse nature of RON-expressing cells, RON has been found to generate multiple outcomes, including the promotion of epithelial-to-mesenchymal transitions, the maintenance of M2 macrophage phenotypes, and the initiation of keratinocyte proliferation and migration. Over aa 25-956, human and mouse RON share 76% aa sequence identity.

• Long Name:

  Macrophage Stimulating Protein Receptor

• Entrez Gene IDs:

  4486 (Human); 19882 (Mouse)

• Alternate Names:

  CD136 antigen; CD136; CDw136c-met-related tyrosine kinase; EC 2.7.10; EC 2.7.10.1; macrophage stimulating 1 receptor (c-met-related tyrosine kinase); MSP R; MSP receptor; MSPR; MST1R; p185-Ron; Protein-tyrosine kinase 8; PTK8 protein tyrosine kinase 8; PTK8; Ron; RONmacrophage-stimulating protein receptor; soluble RON variant 1; soluble RON variant 2; soluble RON variant 3; soluble RON variant 4