• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of Neuro?2A mouse neuroblastoma cells.

  When 5 x 10⁴ cells/well are added to recombinant human NCAM-1 coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour incubation at 37 °C.  The ED₅₀ for this effect is 0.5-3 μg/mL. 

  Optimal dilutions should be determined by each laboratory for each application.

• Source

  Mouse myeloma cell line, NS0-derived

  Human NCAM-1 (Leu20 - Pro603) Accession # NP_001070150	R	Human NCAM-1 (Glu636 - Asn741) Accession # NP_001070150
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Leu20

• Predicted Molecular Mass

  76.5 kDa

• SDS-PAGE

  120 kDa, reducing conditions

Background: NCAM-1/CD56

Neural cell adhesion molecule 1 (NCAM-1) is a multifunctional member of the Ig superfamily. It belongs to a family of membrane-bound glycoproteins that are involved in Ca⁺⁺ independent cell matrix and homophilic or heterophilic cell-cell interactions. NCAM-1 specifically binds to heparan sulfate proteoglycans (1), the extracellular matrix protein agrin (2), and several chondroitin sulfate proteoglycans that include neurocan and phosphocan (3). There are three main forms of human NCAM-1 that arise by alternate splicing. These are designated NCAM-120/NCAM-1 (761 amino acids [aa]), NCAM?140 (848 aa), and NCAM-180 (1120 aa). NCAM-120 is GPI-linked, while NCAM?140 and NCAM-180 are type I transmembrane glycoproteins (4 - 6). Additional alternate splicing adds considerable diversity to all three forms, and extracellular proteolytic processing is possible for NCAM-180 (7 ? 8). NCAM-1 is synthesized as a 761 aa preproprecursor that contains a 19 aa signal sequence, a 722 aa GPI-linked mature region, and a 20 aa C-terminal prosegment (4). The molecule contains five C-2 type Ig-like domains and two fibronectin type-III domains. Human to mouse, NCAM-1 is 93% aa identical. NCAM-1 appears to be highly sialylated. The polysialyation of NCAM-1 reduces its adhesive property and increases its neurite outgrowth promoting features (9). NCAM-1 in the adult brain shows a decline of sialylation relative to earlier developmental periods. In regions that retain a high degree of neuronal plasticity, however, the adult brain continues to express polysialylation-NCAM-1, suggesting sialylation of NCAM-1 is involved in regenerative processes and synaptic plasticity (10 - 13).

• References:

1. Burg, M.A. et al. (1995) J. Neurosci. Res. 41:49.

2. Storms, S.D. and U. Rutishauser (1998) J Biol. Chem. 273:27124.

3. Margolis, R.K. et al. (1996) Perspect. Dev. Neurobiol. 3:273.

4. Dickson, G. et al. (1987) Cell 50:1119.

5. Lanier, L.L. et al. (1991) J. Immunol. 146:4421.

6. Hemperly, J.J. et al. (1990) J. Mol. Neurosci. 2:71.

7. Rutishauser, U.and C. Goridis (1986) Trends Genet. 2:72.

8. Vawter, M.P. et al. (2001) Exp. Neurol. 172:29.

9. Rutihauser, U. (1990) Adv. Exp. Med. Biol. 265:179.

10. Becker, C.G. et al. (1996) J. Neurosci. Res. 45:143.

11. Doherty, P. et al. (1995) J. Neurobiol. 26:437.

12. Eckardt, M. et al. (2000) J. Neurosci. 20:5234.

13. Muller, D. et al. (1996) Neuron 17:413.

• Long Name:

  Neural Cell Adhesion Molecule

• Entrez Gene IDs:

  4684 (Human); 17967 (Mouse); 24586 (Rat)

• Alternate Names:

  CD56 / NCAM-1; CD56 antigen; CD56; MSK39; NCAM1; N-CAM-1; NCAM-1; NCAMantigen recognized by monoclonal 5.1H11; neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM