• Purity

  >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured in a cell proliferation assay using T11 mouse plasmacytoma cells. Nordan, R.P.     et al. (1987) J. Immunol.     139:813. The ED    ₅₀ for this effect is 0.5-2 μg/mL in the presence of 0.75 ng/mL Recombinant Mouse IL?11 (Catalog # )

• Source

  Mouse myeloma cell line, NS0-derived

  Mouse IL-11 R alpha (Met-Gln367) Accession # NP_001156873	DIEGRDP	Mouse IgG2A (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Cys26

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  64.6 kDa (monomer)

• SDS-PAGE

  75-80 kDa, reducing conditions

Background: IL-11 R alpha

Interleukin?11 receptor alpha (IL?11 R alpha, IL?11 R alpha 1) originally designated NR1 in mouse, is a 49 kDa type I transmembrane protein that is a member of the gp130 subfamily of the hematopoietic cytokine receptor family (1?4). Mouse IL?11 R alpha cDNA encodes 432 amino acids (aa) including a 23 aa signal peptide, a 349 aa extracellular domain (ECD) that contains a C2 type Ig?like domain, two fibronectin type III domains, two potential glycosylation sites and a WSXWS motif, a 21 aa transmembrane region and a short (39 aa) cytoplasmic domain (4). Mouse IL?11 R alpha shares 84%, 95%, 85% and 82% aa sequence identity with human, rat, equine and bovine IL?11 R alpha, respectively. In mouse, a second IL?11 R (called alpha 2 or beta ) that differs by only 4 aa is expressed primarily in the testes (5). IL-11 R alpha first binds IL?11 with low affinity, then forms a high affinity receptor when complexed with gp130 homodimers (1, 3). IL?11 R alpha is widely expressed in adults, embryos and embryonic stem cells (4?6). Deletion in female mice causes faulty decidualization and lack of decidual NK cells and results in infertility (7?9). IL-11 is anti-apoptotic for oligodendrocytes, and lack of IL?11 R alpha increases the severity of experimental autoimmune encephalitis (10, 11). IL?11 R alpha is also anti-apoptotic for colonic epithelia, and increased IL?11 signaling may be a factor in inflammation-associated gastrointestinal cancer development (3, 12). IL?11 R alpha enhances osteoclast differentiation and bone remodeling, but inhibits adipocyte differentiation (1, 2). Recombinant soluble IL?11 R alpha confers IL?11 responsiveness to cells expressing gp130, while in cells expressing transmembrane IL?11 R alpha and gp130, soluble IL?11 R alpha acts as an IL?11 antagonist (13?15).

• References:

1. Sims, N.A. and N.C. Walsh (2010) BMB Reports 43:513.

2. White, U.A. and J.M. Stephens (2011) Curr. Pharm. Des. 17:340.

3. Kiessling, S. et al. (2004) J. Biol. Chem. 279:10304.

4. Hilton, D.J. et al. (1994) EMBO J. 13:4765.

5. Bilinski, P. et al. (1996) Biochem. J. 320:359.

6. Davidson, A.J. et al. (1997) Stem Cells 15:119.

7. Paiva, P. et al. (2009) Cytokine Growth Factor Rev. 20:319.

8. Robb, L. et al. (1998) Nat. Med. 4:303.

9. Ain, R. et al. (2004) Dev. Dyn. 231:700.

10. Gurfein, B.T. et al. (2009) J. Immunol. 183:4229.

11. Zhang, J. et al. (2011) J. Immunol. 187:1129.

12. Putoczki, T. and M. Ernst (2010) J. Leukoc. Biol. 88:1109.

13. Curtis, D.J. et al. (1997) Blood 90:4403.

14. Baumann, H. et al. (1996) J. Immunol. 157:284.

15. Karow, J. et al. (1996) Biochem. J. 318:489.

• Long Name:

  Interleukin 11 Receptor alpha

• Entrez Gene IDs:

  3590 (Human); 16157 (Mouse)

• Alternate Names:

  AI314697; GP130; IL-11 R alpha; IL11R alpha; IL11RA; IL-11Ra; Il11ra2; NR1