詳細說明
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized rrUNC5H2/Fc Chimera at 5 μg/mL (100 μL/well) can bind rcNetrin-1 with a linear range of 6-400 ng/mL.
Source
Mouse myeloma cell line, NS0-derived
Chicken Netrin-1
(Gly26 - Ala606)
Accession # Q90922DIEGRGGGSGGGSGGGS 10-His tag N-terminus C-terminus Accession #
N-terminal Sequence
AnalysisGly26
Predicted Molecular Mass
66.3 kDa
SDS-PAGE
75-85 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
128-N1 |
| 128-N1/CF |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. | Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | |
Reconstitution Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. | Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | |
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Netrin-1
Chicken Netrin-1 is the prototypical member of an ever-expanding, laminin-related family of axon-guidance molecules collectively referrred to as netrins (netr is Sanskrit for “one who guides”). The molecule’s cDNA encodes a 606 amino acid (aa) protein precursor that has structural similarity to the N-terminus of the B2 or gamma -chain of laminin. It contains one 250 aa type VI globular domain, three type V (~55 aa) cysteine/glycine rich EGF repeats, and one unique 140 aa “C” domain that binds heparin. Chick Netrin-1 shares 78% aa identity with chicken Netrin-2 and 86% aa identity with mouse and human Netrin-1. Although only two chick netrins are known, the number of known mammalian netrins is increasing. Human and mouse Netrin-3/NTN-2L, and a mouse Netrin-4, that shares homology with the B1 or beta -chain of laminin, have been reported. Cells reported to express Netrin-1 in the embryo include cells of the spinal cord floor plate and somite, cells of the ganglionic eminence, and cells of the floor plate of the met- and caudal mesencephalon. In the adult, neurons of the thalamus, neocortex, and hippocampus, plus Schwann cells, osteoclasts and osteoblasts all reportedly produce Netrin-1. The DCC (deleted in colorectal cancer) gene product as well as the UNC5 family of receptors and the adenosine A2b receptor have been proposed to be functional receptors for Netrin-1.
References:
Serafini, T. et al. (1994) Cell 78:409.
Kennedy, T.E. et al. (1994) Cell 78:425.
Kappler, J. et al. (2000) Biochem. Biophys. Res. Commun. 271:287.
MacLennan, A.J. et al. (1997) J. Neurosci. 17:5466.
Koch, M. et al. (2000) J. Cell. Biol. 151:221.
Corset, V. et al. (2000) Nature 407:747.
Entrez Gene IDs:
9423 (Human); 18208 (Mouse); 396389 (Chicken)
Alternate Names:
netrin 1; Netrin1; Netrin-1; NTN1; NTN1Lnetrin 1, mouse, homolog of
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