• Purity

  >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to bind CD155 in a functional ELISA.

• Source

  Mouse myeloma cell line, NS0-derived

  Human DNAM-1/CD226 (Glu19-Asn247) Accession # Q15762	HIEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Glu19

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  53 kDa (monomer)

• SDS-PAGE

  90 kDa, reducing conditions

Background: DNAM-1/CD226

DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily (1). Mature human DNAM-1 contains a 236 amino acid (aa) extracellular domain (ECD) with two Ig-like C2-set domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins (1, 2). Within the ECD, human DNAM-1 shares 50% and 52% aa sequence identity with mouse and rat DNAM-1, respectively. DNAM-1 is expressed on multiple lymphoid and myeloid cells and interacts with CD155/PVR and Nectin-2/CD112 (3, 4). Ligation of DNAM-1 promotes the activation of NK cells, CD8⁺ T cells, and mast cells (2-6), dendritic cell maturation, megakaryocyte and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; it inhibits the forrmation of osteoclasts (7-10). Platelet-endothelium interactions mediated by DNAM-1 enable the metastasis of tumor cells to the lung (11). In activated, but not in resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to the tyrosine and serine phosphorylation of DNAM-1 during activation (6, 9, 12-14).

• References:

1. Fuchs, A. and M. Colonna (2006) Semin. Cancer Biol. 16:359.

2. Shibuya, A. et al. (1996) Immunity 4:573.

3. Bottino, C. et al. (2003) J. Exp. Med. 198:557.

4. Tahara-Hanaoka, S. et al. (2004) Int. Immunol. 16:533.

5. Dardalhon, V. et al. (2005) J. Immunol. 175:1558.

6. Bachelet, I. et al. (2006) J. Biol. Chem. 281:27190.

7. Reymond, N. et al. (2004) J. Exp. Med. 199:1331.

8. Kakehi, S. et al. (2007) Mol. Cell. Biochem. 301:209.

9. Kojima, H. et al. (2003) J. Biol. Chem. 278:36748.

10. Tahara-Hanaoka, S. et al. (2006) Blood 107:1491.

11. Morimoto, K. et al. (2007) Oncogene July 16 epub.

12. Shibuya, K. et al. (1999) Immunity 11:615.

13. Shibuya, K. et al. (2003) J. Exp. Med. 198:1829.

14. Shibuya, A. et al. (1998) J. Immunol. 166:1671.

• Long Name:

  DNAX Accessory Molecule 1

• Entrez Gene IDs:

  10666 (Human); 225825 (Mouse); 307199 (Rat); 102117316 (Cynomolgus Monkey)

• Alternate Names:

  CD226 antigenplatelet and T cell activation antigen 1; CD226 molecule; CD226; DNAM1; DNAM-1; DNAM1adhesion glycoprotein; DNAM-1DNAX accessory molecule-1; DNAX accessory molecule 1; PTA1; T lineage-specific activation antigen 1 antigen; TLiSA1