• Purity

  >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. When Recombinant Human SIRP alpha /CD172a Fc Chimera (Catalog # ) is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Human CD47 that produces 50% of the optimal binding response is 0.04-0.2 μg/mL.

• Source

  Mouse myeloma cell line, NS0-derived Gln19-Pro139, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  No results obtained. Gln19 inferred from enzymatic pyroglutamate treatment revealing Leu20

• Predicted Molecular Mass

  15 kDa

• SDS-PAGE

  37-45 kDa, reducing conditions

Data Images

Background: CD47

CD47, also known as Integrin-Associated Protein (IAP) and OA3, is a 40-60 kDa variably glycosylated atypical member of the immunoglobulin superfamily (1, 2). Human CD47 is an integral membrane protein that consists of a 123 amino acid (aa) extracellular domain (ECD) with a single Ig-like domain, five membrane-spanning regions with short intervening loops, and a 34 aa C-terminal cytoplasmic tail (3). Alternate splicing of human CD47 generates additional isoforms with deletions in the cytoplasmic tail (3). Within the N-terminal ECD, human CD47 shares 63% aa sequence identity with mouse and rat CD47. A portion of the N-terminal ECD can by shed from smooth muscle cells by MMP-2-mediated proteolysis (4). The ubiquitously expressed CD47 binds to SIRP family members on macrophages, neutrophils, and T cells (5, 6). These interactions prevent macrophage-mediated clearance of healthy CD47-expressing cells and promote immune cell transmigration across the vascular endothelium (5-8). The CD47-SIRP alpha interaction is species specific, and this lack of cross-species interaction has been implicated in xenotransplantation rejection (16). CD47 associates in cis with Fas on T cells and enhances Fas?mediated apoptosis; its ligation promotes T cell anergy and dampens Th1 immune responses (9-11). CD47 also associates in cis with Integrins alpha 4 beta 1, alpha V beta 3, alpha 2b beta 3, and alpha 2 beta 1 which can positively or negatively modulate Integrin?mediated function (2, 12). In the vasculature, CD47 binding by Thrombospondin-1 inhibits the angiogenic and vasorelaxant effects of nitric oxide (2, 13, 14). On dendritic cells and myeloma cells, CD47 ligation by TSP-1 induces giant cell formation and osteoclast differentiation (15).

• References:

1. Sarfati, M. et al. (2009) Curr. Drug Targ. 9:842.

2. Isenberg, J.S. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:615.

3. Campbell, I.G. et al. (1992) Cancer Res. 52:5416.

4. Maile, L.A. et al. (2008) Mol. Endocrinol. 22:1226.

5. Oldenborg, P.-A. et al. (2000) Science 288:2051.

6. Liu, Y. et al. (2002) J. Biol. Chem. 277:10028.

7. Stefanidakis, M. et al. (2008) Blood 112:1280.

8. de Vries, H.E. et al. (2002) J. Immunol. 168:5832.

9. Manna, P.P. et al. (2005) J. Biol. Chem. 280:29637.

10. Avice, M.-N. et al. (2001) J. Immunol. 167:2459.

11. Bouguermouh, S. et al. (2008) J. Immunol. 180:8073.

12. Barazi, H.O. et al. (2002) J. Biol. Chem. 277:42859.

13. Isenberg, J.S. et al. (2009) Nitric Oxide 21:52.

14. Isenberg, J.S. et al. (2009) Matrix Biol. 28:110.

15. Kukreja, A. et al. (2009) Blood 114:3413.

16. Wang H. et al. (2007) Blood 109:836.

• Entrez Gene IDs:

  961 (Human); 16423 (Mouse); 102139846 (Cynomolgus Monkey)

• Alternate Names:

  antigen identified by monoclonal 1D8; Antigenic surface determinant protein OA3; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 antigen; CD47 glycoprotein; CD47 molecule; CD47; IAP; IAPintegrin associated protein; Integrin-associated protein; leukocyte surface antigen CD47; MER6integrin-associated signal transducer; OA3; Protein MER6; Rh-related antigen