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Human/Mouse Shisa-4 Affinity Purified Polyclonal Ab 25 UG

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Human/Mouse Shisa-4 Affinity Purified Polyclonal Ab 25 UG信息二維碼

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產(chǎn)品介紹

    基本參數(shù)

    詳細(xì)說明

    • Species Reactivity

      Human, Mouse

    • Specificity

      Detects human and mouse Shisa?4 in Western blots.

    • Source

      Polyclonal Sheep IgG

    • Purification

      Antigen Affinity-purified

    • Immunogen

      E. coli-derived recombinant human Shisa?4    
      Glu29-Thr85    
      Accession # Q96DD7

    • Formulation

      Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

    • Label

      Unconjugated

    Applications

    • Recommended    
      Concentration

      Sample

    • Western Blot

      1 μg/mL

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Western Blot      
         

    Detection of Human Shisa?4 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 1 μg/mL of Sheep Anti-Human/Mouse Shisa?4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6365) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # ). A specific band was detected for Shisa?4 at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using .

    Preparation and Storage

    • Reconstitution

      Reconstitute at 0.2 mg/mL in sterile PBS.

    • Shipping

      The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

    • Stability & Storage

      Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

      • 12 months from date of receipt, -20 to -70 °C as supplied.

      • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

      • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    Background: Shisa-4

    • Entrez Gene IDs:

      149345 (Human); 77552 (Mouse); 360848 (Rat)

    • Alternate Names:

      C1orf40; chromosome 1 open reading frame 40; FLJ18233; hShisa4; MGC129812; MGC129813; protein shisa-4; shisa homolog 4 (Xenopus laevis); Shisa4; Shisa-4; TMEM58; Transmembrane protein 58MGC102893



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