詳細說明
概述
產(chǎn)品名稱Anti-Pellino 1 + Pellino 2抗體[EPR19282]
描述
兔單克隆抗體[EPR19282] to Pellino 1 + Pellino 2
經(jīng)測試應用WB
種屬反應性
與反應: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Pellino 1 aa 150-250. The exact sequence is proprietary.
Database link: Q96FA3陽性對照
WB: Human Pellino 1 and Pellino 2 full length recombinant proteins; Ramos, PC-12, Jurkat, Daudi, HeLa, C6 and NIH/3T3 whole cell lysates; RAW 264.7 treated with 1 μg/ml LPS for 6 hours whole cell lysate; Mouse and Rat brain lysates.
常規(guī)說明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb? technology. RabMAb? technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放說明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存儲溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA純度Protein A purified
克隆單克隆
克隆編號EPR19282
同種型IgG
研究領域
Epigenetics and Nuclear Signaling
Cell cycle
Kinases/Phosphatases
Other
Signal Transduction
Signaling Pathway
Nuclear Signaling
NFkB Pathway
Signal Transduction
Protein Phosphorylation
Ser / Thr Kinases
Other Kinases
Signal Transduction
Protein Phosphorylation
Ser / Thr Kinases
MAPK Pathway
Anti-Pellino 1 + Pellino 2 antibody [EPR19282] 圖像
Western blot - Anti-Pellino 1 antibody [EPR19282] (ab192833)
All lanes : Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833) at 1/5000 dilution
Lane 1 : Human Pellino 1 full length recombinant protein
Lane 2 : Human Pellino 2 full length recombinant protein
Lane 3 : Human Pellino 3 full length recombinant protein
Lysates/proteins at 0.01 μg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size : 46 kDa
Observed band size : 46 kDa
Blocking/dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1:1 second; Lane 2 and 3:1 minute.
Human Pellino 1 full length recombinant protein contains aa1-418 with a His-Tag?. Human Pellino 2 full length recombinant protein contains aa1-420 with a His-Tag?. Human Pellino 3 full length recombinant protein contains aa1-469 with a His-Tag?. All three recombinant proteins were made in-house.
Western blot - Anti-Pellino 1 antibody [EPR19282] (ab192833)
All lanes : Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833) at 1/1000 dilution
Lane 1 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
Lane 2 : Untreated RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) treated with 1 μg/ml LPS for 6 hours, whole cell lysate
Lane 4 : Untreated PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) treated with 1 μg/ml LPS for 6 hours whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size : 46 kDa
Observed band size : 46 kDa
Exposure time : 3 minutesBlocking/dilution buffer: 5% NFDM/TBST.
The expression of Pellino 1 can be induced by LPS. (PMID: 21204785).
Western blot - Anti-Pellino 1 antibody [EPR19282] (ab192833)
All lanes : Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833) at 1/1000 dilution
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Daudi (Human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 10 μg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size : 46 kDa
Observed band size : 46 kDa
Exposure time : 3 minutesBlocking/dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Pellino 1 antibody [EPR19282] (ab192833)
All lanes : Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 10 μg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size : 46 kDa
Observed band size : 46 kDa
Exposure time : 3 minutesBlocking/dilution buffer: 5% NFDM/TBST.