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Human RB1 MAb (Clone 607121) 25 UG

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Human RB1 MAb (Clone 607121) 25 UG信息二維碼

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產(chǎn)品介紹

    基本參數(shù)

    詳細(xì)說明

    • Species Reactivity

      Human

    • Specificity

      Detects human RB1 in Western blots.

    • Source

      Monoclonal Mouse IgG    1 Clone # 607121

    • Purification

      Protein A or G purified from hybridoma culture supernatant

    • Immunogen

      E.coli-derived recombinant human RB1    
      Lys240-Asn406    
      Accession # P06400

    • Formulation

      Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

    • Label

      Unconjugated

    Applications

    • Recommended    
      Concentration

      Sample

    • Western Blot

      0.1 μg/mL

      See below


    • Simple Western

      1 μg/mL

      See below


    • Immunocytochemistry

      8-25 μg/mL

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Western Blot      
         

    Detection of Human RB1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Daudi human Burkitt's lymphoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF Membrane was probed with 0.1 μg/mL of Human RB1 Monoclonal Antibody (Catalog # MAB6495) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ). A specific band was detected for RB1 at approximately 120 kDa (as indicated). This experiment was conducted under non-reducing conditions and using .

    Immunocytochemistry      
         

    RB1 in MCF?7 Human Cell Line. RB1 was detected in immersion fixed MCF?7 human breast cancer cell line using Human RB1 Monoclonal Antibody (Catalog # MAB6495) at 10 μg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights? 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # ) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for .

    Simple Western      
         

    Detection of Human RB1 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for RB1 at approximately 120 kDa (as indicated) using 1 μg/mL of Mouse Anti-Human RB1 Monoclonal Antibody (Catalog # MAB6495). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

    Preparation and Storage

    • Reconstitution

      Sterile PBS to a final concentration of 0.5 mg/mL.

    • Shipping

      The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

    • Stability & Storage

      Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

      • 12 months from date of receipt, -20 to -70 °C as supplied.

      • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

      • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    Background: RB1

    Retinoblastoma 1 protein (RB-1; also retinoblastoma-associated protein, pp110, and p105-Rb) is a 110 kDa tumor suppressor gene and member of the retinoblastoma protein family.  Human RB-1 is 928 amino acids in length.  The protein contains a Pocket domain (aa 373-771), which is comprised of two other domains, domain A (aa 373-573) and domain B (aa 640-771), and a “spacer” (aa 580-639).  The Pocket domain binds to threonine-phosphorylated domain C (aa 771-928), which thereby prevents interaction with heterodimeric E2F/DP transcription factor complexes.  Human RB-1 is 90% aa identical to mouse RB-1.  RB-1 is expressed in the retina.  The underphosphorylated, active form of RB-1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest.  Defects in RB-1 lead to the childhood cancer retinoblastoma.  

    • Long Name:

      Retinoblastoma-associated protein

    • Entrez Gene IDs:

      5925 (Human)

    • Alternate Names:

      OSRC; osteosarcoma; p105-Rb; pp110; pRb; RB; RB1; retinoblastoma 1; retinoblastoma suspectibility protein; retinoblastoma-associated protein



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