• Species Reactivity

  Human

• Specificity

  Detects human APC C-Terminus in direct ELISAs and Western blots.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant human APC    
  Ser2537-Val2843    
  Accession # P25054

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 μg/mL

  Recombinant Human APC C-Terminus

• 
  

• Immunohistochemistry

  5-15 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APC

APC is a ubiquitous, tumor-suppressor protein that is mutated in most colon cancers, generally resulting in a gene product that is truncated in the carboxy terminal region. APC contains several binding domains. The region used as an immunogen overlaps the EB1, DLG (PDZ sequence), and PSD-93 domains. These domains bind microtubules, mitotic spindles, and F-actin and are involved in cell cycle, division, and migration. The immunogen does not include the beta -catenin-binding region, which antagonizes Wnt signaling. The human APC amino acid sequence used as immunogen is 83% and 86% identical to mouse and rat APC, respectively.

• Long Name:

  Adenomatosis Polyposis Coli

• Entrez Gene IDs:

  324 (Human); 11789 (Mouse); 24205 (Rat)

• Alternate Names:

  adenomatosis polyposis coli tumor suppressor; adenomatosis polyposis coli; adenomatous polyposis coli protein; adenomatous polyposis coli; APC; BTPS2; Deleted in polyposis 2.5; DP2; DP2.5DP2; DP3; FPC; GS; Min; Protein APC