• Species Reactivity

  Human

• Specificity

  Detects human DLL1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) DLL3 or rhDLL4 is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 251123

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human DLL1    
  Ser22-Gly540    
  Accession # AAG09716

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Immunohistochemistry

  15-30 μg/mL

  Immersion fixed paraffin-embedded sections of human squamous cell carcinoma

• 
  

• Immunocytochemistry

  8-25 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DLL1

Delta-like protein 1 (DLL1) is a 90?100 kDa type I transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature human DLL1 consists of a 528 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23 aa transmembrane segment, and a 155 aa cytoplasmic domain (1). Within the ECD, human DLL1 shares 91% aa sequence identity with mouse and rat DLL1. It shares 26%, 37%, and 54% aa sequence identity with DLL2, 3, and 4, respectively. A 60 kDa ECD fragment released by ADAM9, 12, or 17 mediated proteolysis, promotes the proliferation of hematopoietic progenitor cells (2, 3). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent gamma -secretase, enabling the cytoplasmic domain to migrate to the nucleus (4). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (5). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, plus B and T lymphocyte differentiation (6?11). The upregulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (12). DLL1 is also overexpressed in cervical carcinoma and glioma and contributes to tumor progression (1, 13).

• References:

1. Gray, G.E. et al. (1999) Am. J. Pathol. 154:785. 

2. Dyczynska, E. et al. (2007) J. Biol. Chem. 282:436. 

3. Karanu, F.N. et al. (2001) Blood 97:1960. 

4. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751. 

5. Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499. 

6. Takahashi, Y. et al. (2003) Development 130:4259. 

7. Teppner, I. et al. (2007) BMC Dev. Biol. 7:68.

8. Kiernan, A.E. et al. (2005) Development 132:4353.

9. Schmitt, T.M. and J.C. Zuniga-Pflucker (2002) Immunity 17:749.

10. Hozumi, K. et al. (2004) Nat. Immunol. 5:638.

11. Santos, M.A. et al. (2007) Proc. Natl. Acad. Sci. 104:15454.

12. Limbourg, A. et al. (2007) Circ. Res. 100:363.

13. Purow, B.W. et al. (2005) Cancer Res. 65:2353.

• Long Name:

  Delta-like 1

• Entrez Gene IDs:

  28514 (Human); 13388 (Mouse); 84010 (Rat)

• Alternate Names:

  delta (Drosophila)-like 1; Delta 1; Delta; Delta1; delta-like 1 (Drosophila); delta-like protein 1; DL1; DLL1; Drosophila Delta homolog 1; H-Delta-1