• Species Reactivity

  Mouse

• Specificity

  Detects mouse IL?3 R alpha /CD123 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human IL-3 R alpha  and recombinant mouse IL-3 R beta is observed.

• Source

  Monoclonal Rat IgG2a Clone # 151231

• Immunogen

  S. frugiperda insect ovarian cell line     Sf 21-derived recombinant mouse IL?3 R alpha /CD123    
  Ser17-Lys331    
  Accession # P26952

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 405

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 μg/10    ⁶ cells

  Mouse splenocytes

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-3 R alpha/CD123

Interleukin 3 (IL-3) is a pleiotropic cytokine produced primarily by activated T cells or mast cells. IL-3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage-committed cells. The biological effects of IL-3 on the various cell types are mediated by the binding of IL-3 to specific cell surface receptor complexes. The functional high-affinity IL-3 receptor is a heterodimer consisting of a ligand binding alpha  subunit and the beta  subunit. The alpha  subunit alone binds IL-3 with low affinity. The beta  subunit is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. The beta  subunit has also been found to be a component of the high-affinity receptor complex for IL-5 and GM-CSF and is also referred to as the beta common ( beta c) chain. In the mouse, there are two IL-3 R beta proteins. The first identified mouse IL-3 R beta was also called AIC2A and binds IL-3 with low affinity (1). The second mIL-3 R beta was referred to as AIC2B (2). AIC2B doesn’t bind IL-3 and is the homolog of the human IL-3 R beta. AIC2A was found to be the result of a gene duplication event. The mouse IL-3 R alpha, also called SUT-1, will form complexes with either mouse IL-3 R beta protein (3). Both the alpha and the beta  subunits are members of the cytokine receptor superfamily.

• References:

1. Itoh, N. et al. (1990) Science 247:324.

2. Gorman, D.M. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5459.

3. Hara, T. and A. Miyajima (1992) EMBO J. 11:1875.

4. Schrader, J.W. (2001) Cytokine Reference, Oppenheim, J.J. and M. Feldmann, eds, Academic Press, New York, p. 1899.

• Long Name:

  Interleukin 3 Receptor alpha

• Entrez Gene IDs:

  3563 (Human); 16188 (Mouse)

• Alternate Names:

  CD123 antigen; CD123; hIL3Ra; hIL-3Ra; IL-3 R alpha; IL-3 receptor subunit alpha; IL3R alpha; IL-3R subunit alpha; IL3R; IL3RA; IL-3Ra; IL-3R-alpha; IL3RAY; IL3RX; IL3RY; interleukin 3 receptor, alpha (low affinity); interleukin-3 receptor subunit alpha; MGC34174