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Human/Mouse Glucagon Alexa Fluor 488 Antibody 100 UG

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Human/Mouse Glucagon Alexa Fluor 488 Antibody 100 UG信息二維碼

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產(chǎn)品介紹

    基本參數(shù)

    詳細(xì)說(shuō)明

    • Species Reactivity

      Human, Mouse

    • Specificity

      Detects human and mouse Glucagon.

    • Source

      Monoclonal Mouse IgG    2A Clone # 181402

    • Purification

      Protein A or G purified from hybridoma culture supernatant

    • Immunogen

      Human Glucagon synthetic peptide

    • Formulation

      Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

    • Label

      Alexa Fluor 488

    Applications

    • Recommended    
      Concentration

      Sample

    • Intracellular Staining by Flow Cytometry

      0.25-1 μg/10    6 cells

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Intracellular Staining by Flow Cytometry      
         

    Detection of Glucagon in beta TC-6 Mouse Cell Line by Flow Cytometry. beta TC-6 mouse beta cell insulinoma cell line was stained with Alexa Fluor? 488-conjugated Mouse Anti-Human/Mouse Glucagon Monoclonal Antibody (Catalog # IC1249G, filled histogram) or isotype control antibody (Catalog # , open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # ) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # ). View our protocol for .

    Preparation and Storage

    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

    • Stability & Storage

      Protect from light.    Do not freeze.    

      • 12 months from date of receipt, 2 to 8 °C as supplied.

    Background: Glucagon

    Glucagon is a 29 amino acid residue pancreatic peptide hormone. It stimulates gluconeogenesis and glycogenolysis to counter the glucose-lowering action of insulin.

    • Entrez Gene IDs:

      2641 (Human); 14526 (Mouse); 24952 (Rat)

    • Alternate Names:

      GCG; glicentin-related polypeptide; Glucagon; GRPP









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