• Species Reactivity

  Human

• Specificity

  Detects human IL?3 R alpha /CD123 in direct ELISAs and Western blots.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 32703

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  S. frugiperda insect ovarian cell line     Sf 21-derived recombinant human IL?3 R alpha /CD123    
  Lys20-Arg305, predicted    
  Accession # P26951

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  PerCP (Peridinin-chlorophyll Protein Complex)

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 μL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-3 R alpha/CD123

IL-3 is a pleiotropic cytokine that can stimulate proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors (1, 2). IL-3 exerts its activity through binding to a specific cell surface receptor known as IL-3 R. IL-3 R is a heterodimeric structure composed of a 70 kDa IL-3 R alpha subunit (CD123) and a 120-140 kDa IL-3 R beta subunit (CD131) (3, 4). IL-3 R alpha binds IL-3 with relatively low affinity. In the presence of IL-3 R beta, however, IL-3 R alpha has a much higher affinity for IL-3. It is not clear how signal transduction occurs following IL-3 binding. The IL-3 R alpha chain has a very short intracellular domain while the IL?3 R beta chain has a very large cytoplasmic domain. The IL?3 R beta chain is also shared by the receptors for IL-5 and GM-CSF. Cells known to express IL-3 receptors include hematopoietic progenitors, epithelial cells, double negative T cells, mast cells, basophils and blood monocytes (5).

• References:

1. Moore, M.A.S. et al. (1991) Blood 72:944.

2. Warren, D.J. et al. (1988) J. Immunol. 140:94.

3. Plant M. et al. (1989) Nature 339:150.

4. Budel, L.M. et al. (1990) Blood 75:1439.

5. Schrader, J.W. et al. (1988) In Interleukin-3: The Panspecific hemopoietin (ed. J.W. Schrader), Academic Press, San Diego, CA.

• Long Name:

  Interleukin 3 Receptor alpha

• Entrez Gene IDs:

  3563 (Human); 16188 (Mouse)

• Alternate Names:

  CD123 antigen; CD123; hIL3Ra; hIL-3Ra; IL-3 R alpha; IL-3 receptor subunit alpha; IL3R alpha; IL-3R subunit alpha; IL3R; IL3RA; IL-3Ra; IL-3R-alpha; IL3RAY; IL3RX; IL3RY; interleukin 3 receptor, alpha (low affinity); interleukin-3 receptor subunit alpha; MGC34174