• Species Reactivity

  Human

• Specificity

  Detects human Complement Component C5a R1. Stains human Complement Component C5a R1 transfected cells but not irrelevant transfectants.

• Source

  Monoclonal Mouse IgG    _2A Clone # 347214

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  NS0 mouse myeloma cell line transfected with human Complement Component C5a R1    
  Met1-Val350    
  Accession # P21730

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 μL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Complement Component C5a R1

Complement Component C5a R1 (C5aR1), also known as C5a ligand and CD88, is a 7TM protein expressed on myeloid, endothelial, epithelial, and smooth muscle cells. C5aR binds the activated complement anaphylatoxin C5a. In established allergic environments, this triggers neutrophil and eosinophil chemotaxis and the release of proinflammatory mediators. In contrast, C5aR1/C5a interactions are protective during allergen sensitization. Human C5aR1 shares 66% amino acid sequence identity with mouse and rat C5aR.

• Long Name:

  Complement Component C5a Receptor 1

• Entrez Gene IDs:

  728 (Human); 12273 (Mouse)

• Alternate Names:

  C5a anaphylatoxin chemotactic receptor; C5A; C5aR; C5a-R; C5AR1; C5ARC5a anaphylatoxin receptor; C5R1C5a ligand; CD88 antigen; CD88; complement component 5 receptor 1 (C5a ligand); complement component 5 receptor 1; complement component 5a receptor 1; Complement Component C5a R1; Complement Component C5aR1