• Purity

  >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3?E1 mouse preosteoblast cells. The ED    ₅₀ for this effect is 60-360 ng/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt?3a (Catalog # ).

• Source

  Mouse myeloma cell line, NS0-derived Thr25-Arg302, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Thr25

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  31 kDa

• SDS-PAGE

  34-43 kDa, reducing conditions

Data Images

Background: Stanniocalcin 2/STC-2

Stanniocalcin 2 (STC-2) is a secreted disulfide-linked homodimeric glycoprotein hormone that is related to the STC protein first discovered from corpuscles of stannius in fish (1). The STC-2 has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is phosphorylated by casein kinase 2 exclusively on its serine residues (2). Its C-terminus contains a cluster of histidine residues which may interact with metal ions (1). STC-2 is expressed in a wide variety of tissues. In the ovary, STC-2 has been shown to be a paracrine hormone that regulates granulosa cell function (1). The amino acid sequence of human mature STC-2 is 36% identical to that of human STC-1. It is also 99% and 88% identical to that of monkey and mouse STC-2, respectively (3). STC-2 enhances mesenchymal stem cell survival (4), promotes cell proliferation in cervical cancer (5), suppresses breast cancer cell migration and invasion (6), promotes osteoblast differentiation (7), and inhibits longitudinal bone growth directly at the growth plate (8). It is also a biomarker for cervical and lung cancers (9, 10),

• References:

1. Luo, C. et al. (2005). Endocrinology 146:469.

2. Tagliabracci, V. S. et al. (2015). Cell 161:1619.

3. Ishibashi, K. et al. (1998). Biochem. Biophys. Res. Commun. 250:252.

4. Kim PH, et al. (2015) BMB Rep. 48:702.

5. Wang Y, et al. (2015) Biochem Biophys Res Commun. 466:362.

6. Hou J, et al. (2015) PLoS One. 10:e0122179.

7. Zhou J, et al. (2016) Mol Med Rep. 14:5653.

8. Wu S, et al. (2006) J Biol Chem. 281: 5120.

9. Shen XJ, et al. (2014) Int J Clin Exp Pathol. 7: 8770.

10. Na SS, et al. (2015) Biochim Biophys Acta. 1854:668.

• Entrez Gene IDs:

  8614 (Human); 20856 (Mouse)

• Alternate Names:

  Stanniocalcin 2; stanniocalcin-2; STC2; STC-2; STC-2Stanniocalcin-related protein; STC-related protein; STCRP