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Recombinant Human CTRP2/C1qTNF2, CF 50 UG

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產(chǎn)品介紹

    基本參數(shù)

    詳細說明

    • Purity

      >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

    • Endotoxin Level

      <0.10 EU per 1 μg of the protein by the LAL method.  

    • Activity

      Measured by its ability to induce phospho AMPK activation in C2C12 mouse differentiated myocytes. 100 ng/mL of Recombinant Human CTRP2/C1qTNF2 induces phosphorylation of AMPK.

    • Source

      Human embryonic kidney cell, HEK293-derived

      DYKDDDDK
      (FLAG tag)
      Human CTRP2/C1qTNF2
      (Gly144-Val285)
      Accession # Q9BXJ5
      GGGGSHuman CTRP2/C1qTNF2
      (Gly144-Val285)
      Accession # Q9BXJ5
      GGGGSHuman CTRP2/C1qTNF2
      (Gly144-Val285)
      Accession # Q9BXJ5
      N-terminus



      C-terminus
    • Accession #

    • N-terminal Sequence    
      Analysis

      Asp (Flag tag)

    • Structure / Form

      GS-linked homotrimer

    • Predicted Molecular Mass

      49 kDa

    • SDS-PAGE

      54-62 kDa, reducing conditions

    9436-TN

     

    Formulation Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl.


    Reconstitution Reconstitute at 200 μg/mL in PBS.



    Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.


    Stability & Storage:       Use a manual defrost freezer and avoid repeated freeze-thaw cycles.      

    • 12 months from date of receipt, -20 to -70 °C as supplied.

    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.


    Data Images

    Bioactivity      


           

    Recombinant Human CTRP2/C1qTNF2 Induces Phosphorylation of AMPK in C2C12 Differentiated Myocytes. C2C12 differentiated myocytes were treated with Recombinant Human CTRP2/C1qTNF2 (Catalog # 9436?TN) for 1 hour prior to Western blot analysis.

    Background: CTRP2/C1qTNF2

    Complement C1q tumor necrosis factor-related protein 2 (C1qTNF2), also known as CTRP-2 or zacrp2 is an approximately 30 kDa member of the C1q family of secreted proteins (1, 2). Like other members of that family, CTRP2 contains a collagen-like region and one C1q-like domain (3). CTRP2 shares 42% amino acids identity with adiponectin at the presumed functional globular domain (4) and is expressed in stromal vascular cells (5). CTRP2 circulates as a trimeric glycoprotein in plasma (6), and can also form homo-oligomer or hetero-oligomer with other CTRPs (7). C1qTNF2 has been shown to phosphorylate AMPK and ACC in skeletal muscle leading to an increase in glycogen accumulation and fatty acid oxidation (8). C1qTNFs are of clinical interest as diagnostic or therapeutic targets in diseases where energy homeostasis is unbalanced such as obesity, insulin resistance, and type 2 diabetes (2).

    • References:

      1. Hosszu, KK,. et al. (2012) Front. Immunol. 5:3.

      2. Seldin, MM. et al. (2014) Rev. Endocr. Metab. Disord. 15:111.

      3. Kim, K.-Y et al. (2006) FEBS Lett. 580:3953.

      4. Wong, GW et al. (2004). Proc. Natl. Acad. Sci. U.S.A. 101:10302.

      5. Sch?ffler A. et al. (2012) Trends in Endocrinology and Metabolism. 23:194.

      6. Wong, GW et al. (2008). Biochem J. 416:161.

      7. Peterson, JMl, et al. (2014). PLOS one 9:e88535.

      8. Sch?ffler A. et al. 2007) Trends in Immunology 28:393.

    • Long Name:

      C1q And Tumor Necrosis Factor Related Protein 2

    • Entrez Gene IDs:

      114898 (Human); 69183 (Mouse); 497886 (Rat)

    • Alternate Names:

      C1q and tumor necrosis factor related protein 2; C1QTNF2; complement-c1q tumor necrosis factor-related protein 2; CTRP2; CTRP2complement C1q tumor necrosis factor-related protein 2; zacrp2

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