• Species Reactivity

  Mouse

• Specificity

  Detects mouse Dtk extracellular domain in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Dtk, recombinant mouse (rm) Mer, or rmAxl is observed.

• Source

  Monoclonal Rat IgG    ₁ Clone # 109646

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse Dtk    
  Ala31-Ser418    
  Accession # P55144

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 488

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  5 μL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Tyro3/Dtk

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a new receptor tyrosine kinase subfamily. The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. The binding affinities for Gas6 is in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain.

• References:

1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.

2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.

• Long Name:

  Tyrosine Protein Kinase Receptor TYRO3

• Entrez Gene IDs:

  7301 (Human); 22174 (Mouse)

• Alternate Names:

  Brt; BYK; Dtk; EC 2.7.10; EC 2.7.10.1; FLJ16467; Rse; Sky; Tif; TYRO3 protein tyrosine kinase; Tyro3; Tyrosine-protein kinase byk; Tyrosine-protein kinase DTK; tyrosine-protein kinase receptor TYRO3; Tyrosine-protein kinase RSE; Tyrosine-protein kinase SKY