• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured in a competitive binding assay. When rmICOS/Fc Chimera (Catalog # 168-CS) is immobilized at 1 μg/mL (100 μL/well), rmB7-H2 inhibits 50% binding of biotinylated rhB7-H2/Fc Chimera (2.5 ng/mL) at the concentration range of 80?120 ng/mL.

• Source

  Mouse myeloma cell line, NS0-derived

  Mouse B7-H2 (Glu47 - Lys279) Accession # Q9JHJ8	DIEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Glu47

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  53 kDa (monomer)

• SDS-PAGE

  80-90 kDa, reducing conditions

Background: B7-H2

Mouse B7-H2, also called B7RP-1, B7h, LICOS, and GL50, is a member of the growing B7 family of immune co?stimulatory proteins. Other family members include B7-1, B7-2, B7-H1 (PD-L1), PD-L2, and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily. The extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Among the family members, they share about 20 - 25% amino acid identity. Mouse and human B7-H2 share approximately 49% amino acid identity. B7-H2 has been identified as the ligand for ICOS, a member of the CD28 family of co?stimulatory receptors. Mouse B7-H2 is a 322 amino acid (aa) protein with a putative 46 aa signal peptide, a 233 aa extracellular domain, a 19 aa transmembrane region, and a 24 aa cytoplasmic domain. Mouse B7-H2 is expressed constitutively on resting B cells and at low levels on monocytes. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and T_h differentiation.

• References:

1. Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.

2. Ling, V. et al. (2000) J. Immunol. 164:1653.

3. Wang, S. et al. (2000) Blood 96:2808.

4. Brodie, D. et al. (2000) Curr. Biol. 10:333.

5. Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.

6. Swallow, M.M. et al. (1999) Immunity 11:423.

7. Yoshinaga, S.K. et al. (1999) Nature 402:827.

• Long Name:

  B7 Homolog 2

• Entrez Gene IDs:

  23308 (Human); 50723 (Mouse); 102132485 (Cynomolgus Monkey)

• Alternate Names:

  B7H2; B7-H2; B7H2B7 homolog 2; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50; B7RP-1LICOS; CD275 antigen; CD275; GL50; GL50transmembrane protein B7-H2 ICOS ligand; ICOSL; ICOS-L; ICOSLG; inducible T-cell co-stimulator ligand; KIAA0653ICOS ligand