• Purity

  >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <1.0 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. Immobilized rmIL-1F5, rmIL-1F6, or rmIL-1F8 at 1 μg/mL (100 μL/well) can bind    
  rmIL-1 Rrp2/his with a linear range of 0.078-5 μg/mL.

• Source

  Mouse myeloma cell line, NS0-derived Asp22-Arg338 (Ala158Val), with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Asp22

• Predicted Molecular Mass

  36.8 kDa

• SDS-PAGE

  55-68 kDa, reducing conditions

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: IL-1 Rrp2/IL-1 R6

IL-1 Rrp2, also known as IL-1 R6, is a member of the IL-1 R/TLR superfamily. Members of this superfamily have a characteristic Toll-IL-1 R (TIR) domain in their cytoplasmic region. This superfamily can be further divided into three subgroups based on extracellular region analogies. IL-1 Rrp2 belongs to Subgroup 1 which is characterized by the presence of at least one immunoglobulin-like domain (1). Mouse IL-1 Rrp2/IL-1R6 is a 70 kDa type I transmembrane glycoprotein that is synthesized as a 574 amino acid (aa) precursor. The precursor contains a 21 aa signal sequence, a 317 aa extracellular region, a 21 aa transmembrane segment, and a 215 aa cytoplasmic domain (2). The extracellular region contains three C-type Ig-like domains. Within the extracellular region, mouse IL-1 Rrp2 is 64% and 80% aa identical to human and rat IL-1 Rrp2, respectively. Expression of IL-1 Rrp2 is restricted to lung epithelium, monocytes, skin-derived keratinocytes, fibroblasts and, to a lesser extent, endothelial cells (3). Studies have demonstrated that the functional receptor for IL-1F6, IL-1F8 and IL-1F9 is a combination of IL-1 Rrp2 and IL-1 RAcP. This suggests that IL-1 Rrp2 ligands may signal in a similar fashion to IL-1 and IL-18. In each case, there is a binding receptor which, upon ligation, recruits a second receptor as a signaling component, forming an active heterodimeric receptor complex (4).

• References:

1. Dunne, A. and L.A. O’Neill, (2003) Sci. STKE Feb 25 (171):re3.

2. Born, T.L. et al. (2000) J. Biol. Chem. 275:29946.

3. Lovenberg, T.S. et al. (1996) J. Neuroimmunol. 70:113.

4. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.

• Long Name:

  Interleukin 1 Receptor-like 2

• Entrez Gene IDs:

  8808 (Human); 107527 (Mouse); 171106 (Rat)

• Alternate Names:

  IL-1 R6; IL-1 Rrp2; IL1RL2; IL-1RL2; IL1Rrp2; IL-1Rrp2